Effects of Diet on Resource Utilization by a Model Human Gut Microbiota Containing Bacteroides cellulosilyticus WH2, a Symbiont with an Extensive Glycobiome
Figure 2
B. cellulosilyticus WH2 CAZyme expression in mice fed different diets.
(A) Overview of the 50 most highly expressed B. cellulosilyticus WH2 CAZymes (GHs, GTs, PLs, and CEs) for samples from each diet treatment group. List position denotes the rank order of gene expression for each treatment group, with higher expression levels situated at the top of each list. Genes common to both lists are identified by a connecting line, with the slope of the line indicating the degree to which a CAZyme's prioritized expression is increased/decreased from one diet to the other. CAZy families in bold, colored letters highlight those list entries found to be significantly up-regulated relative to the alternative diet (i.e., a CAZyme with a bold green family designation was up-regulated on the LF/HPP diet; a bold orange family name implies a gene was up-regulated significantly on the HF/HS diet). Statistically significant fold-changes between diets are denoted in the “F.C.” column (nonsignificant fold-changes are omitted for clarity). (B) Breakdown by CAZy family of the top 10% most expressed CAZymes on each diet whose expression was also found to be significantly higher on one diet than the other. Note that for each diet, the family with the greatest number of up-regulated genes was also exclusively up-regulated on that diet (LF/HPP, GH43; HF/HS, GH13). In total, 25 genes representative of 27 families and 12 genes representative of 13 families are shown for the LF/HPP and HF/HS diets, respectively.