Neto1 Is a Novel CUB-Domain NMDA Receptor–Interacting Protein Required for Synaptic Plasticity and Learning
Figure 7
Neto1 Loss of Function Decreases tbLTP in CA1 Hippocampus
(A) fEPSP slope and (B) fiber volley amplitude plotted as a function of stimulus intensity in Neto1+/+ (+/+, open circles) and Neto1-null (tlz/tlz, filled circles) mice. Strength of Schaffer collateral stimulation is indicated on the horizontal axis. Representative traces show fiber volley and fEPSPs (scale bars: 2 ms, 1.5 mV).
(C) Paired-pulse facilitation of fEPSPs in slices from +/+ and tlz/tlz mice. Interstimulus interval is indicated on the horizontal axis. P1, fEPSP slope first response; P2, fEPSP slope second response.
(D) Summary scatter plot shows grouped normalized fEPSP slope every 1 min in slices from +/+ (n = 20 slices) and tlz/tlz (n = 17 slices; **, p < 0.01; ***, p < 0.001 versus +/+) mice. Theta-burst stimulation (TBS) was delivered to Schaffer collateral CA1 synapses at the 30-min time point. fEPSP slope was normalized to the mean slope of fEPSPs recorded during the 10-min period immediately before TBS. Inset: average of six consecutive fEPSPs recorded at the times indicated before or after theta-burst stimulation (a or b, respectively; scale bars: 15 ms, 0.4 mV). Error bars show ± standard error of the mean (SEM).