Drug-Induced Epigenetic Changes Produce Drug Tolerance
Figure 4
The Sodium Butyrate–Induced Benzyl Alcohol Resistance Phenotype Is Dependent upon a Functional slo Gene
(A) The consumption of sodium butyrate causes wild-type Canton S (CS) flies to acquire BA resistance. The label 0MSB indicates flies fed normal food, and 0.05MSB represents flies fed food supplemented with 0.05 M sodium butyrate.
(B) Sodium butyrate consumption does not cause slo4 homozygotes to acquire BA resistance.
(C) ash218/slo4 transheterozygotes also did not show sodium butyrate–induced BA resistance.
(D) However, slo4/+ flies show a sodium butyrate–inducible resistance to BA sedation. The period of sedation in (A) and (B) versus (C) and (D) is somewhat different. This is likely the product of slight differences in the dose of the drug because the (A–B) pair and the (C–D) pair were performed on different days. Significance between recovery curves was determined by the log-rank test (n = 45; a single asterisk [*] indicates p < 0.05, and double asterisks [**] indicate p < 0.01).