Plasticity of the cis-Regulatory Input Function of a Gene
Figure 2
The Wild-Type lac Promoter Region and the Point Mutations Used in This Study
Locations of point mutations are indicated by dots, where a red indicates mutations used in all variants, black means change to A or T with equal probability, and white means change to A, C, or T with equal probability. Also shown are the positions of the two LacI sites O1 and O3, the CRP site, and the RNAp site (−10 and −35 regions). Note that the promoter region is displayed in this figure in two parts: the sequence in the top part ends just downstream of the beginning of the sequence in the bottom part. The black arrow indicates the transcription start site of the lacZ gene.