Many Ribosomal Protein Genes Are Cancer Genes in Zebrafish
Figure 1
Spindle Cell Tumors Resembling MPNSTs in Zebrafish Heterozygous for Mutations in RP Genes
(A and B) Fish with apparent masses, as indicated by the arrows, or other evident pathology were selected for histological analysis: (A) a hi2582 fish, (B) a hi1034B fish.
(C–H) Histopathology of representative tumors stained with hematoxylin and eosin reveals patterns consistent with the diagnosis of MPNST in hi10 fish (C and D), hi1974 fish (E–G), and hi1807 fish (H).
(C) Tumors typically filled the entire abdomen (sb, swim bladder; br, brain) (80×).
(D) A large tumor with central necrosis is seen emanating from the optic nerve (n) (e, eye) (20×).
(E) Tumors consist of spindle cells that stack into short fascicles, typically organizing into whorls (400×).
(F) Tumor is aggressively invading muscle (m) and gill (g) (br, brain) (100×).
(G) Mitotic figures (arrows) are evident (1000×).
(H) Areas of focal necrosis (arrows) are frequently seen (200×).