A Protein Complex Containing the Conserved Swi2/Snf2-Related ATPase Swr1p Deposits Histone Variant H2A.Z into Euchromatin
Figure 3
Swc4p and Bdf1p Were Components of SWR1-Com
This figure shows immunoblots of analytical-scale TAP purifications. The captured TAP-tagged protein is indicated above the gels, and the protein that was tested for association is indicated at the right side.
(A) Association of Swc4p and Tra1p. Swc4-HA was present in purifications from Yaf9-TAP, Esa1-TAP, Rvb2-TAP, and Swr1-TAP but not Ino80-TAP. NuA4 was only present in the Yaf9-TAP and Esa1-TAP material.
(B) Reciprocal confirmation of Swc4p being part of NuA4. Swc4-TAP and Yaf9-TAP purified material contained NuA4 components Esa1p and Tra1p.
(C) Association of Bdf1p. Bdf1p was present in purifications from Swr1-TAP, Yaf9-TAP, and Swc4-TAP but not Esa1-TAP.