Title
Data for: Using the Daphnia magna Transcriptome to Distinguish Water Source: Wetland and Stormwater Case Studies
Published Date
2022-07-18
Authors
Author Contact
Jankowski, Mark (jankowski.mark@epa.gov)
Type
Dataset
Experimental Data
Genomics Data
Abstract
A major challenge in ecotoxicology is accurately and sufficiently measuring chemical exposures and biological effects given the presence of complex and dynamic contaminant mixtures in surface waters. Our study examined the performance of the Daphnia magna transcriptome to detect distinct responses across three water sources in Minnesota: laboratory [well] waters, wetland waters, or stormwaters. Pyriproxyfen (PPF) was included as a gene expression and male neonate production positive control to examine whether gene expression resulting from exposure to this well-studied juvenoid hormone analog can be detected in complex matrices. Laboratory-reared (<24 hr) D. magna were exposed to a water source and/or PPF for 16 d to monitor phenotypic changes or 96 hr to examine gene expression responses using Illumina HiSeq 2500 (10 million reads per library, 50-bp paired-end (2x50)).
Description
Data herein are associated with figures and tables in DOI 10.1002/etc.5392. Raw and processed RNA sequencing data can be found in NCBI GEO (https://www.ncbi.nlm.nih.gov/geo/). R code used to analyze the data can be found in GitHub (https://github.com/jankow55/Daphnia-bioassay.git).
Funding information
Sponsorship:
U.S. Environmental Protection Agency: Clean Water Act Section 106 Monitoring Funds; State of Minnesota: Clean Water Fund of Minnesota's Clean Water and Land
Referenced by
Jankowski, M.D., Fairbairn, D.J., Baller, J.A., Westerhoff, B.M. and Schoenfuss, H.L. (2022), Using the Daphnia magna Transcriptome to Distinguish Water Source: Wetland and Stormwater Case Studies. Environ Toxicol Chem. Accepted Author Manuscript.
Related to
NCBI GEO
Github Repository
License
CC0 1.0 Universal
Suggested Citation
Jankowski, Mark D; Fairbairn, David J; Baller, Joshua A; Westerhoff, Benjamin M; Schoenfuss, Heiko L.
(2022). Data for: Using the Daphnia magna Transcriptome to Distinguish Water Source: Wetland and Stormwater Case Studies.
Retrieved from the Data Repository for the University of Minnesota,
https://doi.org/10.13020/4hq3-v890.