The aim of present study is to fabricate a micro pillar from polycrystalline Ti plate, to perform a compression examination for a micro polycrystalline Ti pillar and to investigate its deformation behavior using Acoustic Emission (AE) technique. First, a micro polycrystalline Ti pillar, which width is several hundred from tens of micrometer, is fabricated by original technique. Then, its crystalline structure is investigated by Electron Back Scatter Diffraction (EBSD) device. Second, in-house micro compressive device is developed, and compressive test is performed for a fabricated micro pillar. In addition, AE signals are measured during compressive loading. As a result, it was seen that a lot of small twin regions occured at early deformation, then small number of large twin region occured with the progress of deformation for present micro Ti pillar.