The healing of articular cartilage that possesses little capacity for self-repair after having been damaged is still a clinical problem. This paper describes the effects of vibration stimulation on chondrocytes in three-dimensional culture that imitates actual articular cartilage in relation to the production of regenerative cartilage tissue, using collagen gel and artificial skin as a carrier. The obtained results are the mechanical vibration advances the matrix production and proliferation of chondrocytes.