A web is continuous, flexible two dimensional material such as paper, plastic film, textile, thin metal etc. then it has various applications in many industries because of their advantage of compactness or anther function. In near future, the market of optical films became very large in some industries such as crystal liquid display, PC, compact IT instrument. On the other hand, it is well known that the web is damaged by wrinkling in transportation of webs. Therefore, it is important to predict and protect the wrinkles however there is no literature treated the effectiveness of anisotropic in Young's modulus of optical film in machine direction and cross machine direction on wrinkles. This paper describes the experimental study on wrinkles or transportation of optical films and the effect of anisotropic of optical films wrinkles are clarified experimentally.