Actin protein, which is one of the cytoskeletal proteins, gathers and polymerizes under the cell membrane, and leads to the formation of the protrusion of the cell membrane. Therefore, actin protein participates greatly in cell movement. In this study, in order to evaluate the actin protein dynamics at the protrusion process of the cell movement quantitatively, movements of actin-labeled cells were observed under the confocal laser scanning microscope, and time-laps images were obtained. Based on image analysis of the formation of actin stress fibers, it was expected that the mesh-work of stress fibers was actively formed in the protrusion parts of the cell during the cell movement. In addition, a number of immobile spots like focal adhesion were observed adjacent to the active mesh-work of stress fibers and their distribution and time between appearance and disappearance points of each spot were analyzed. As a result, it was presumed that immobile spots close to the centroid of the cell during movement have a crucial role to drive cell movement as an anchorage.