The weld pass sequence generally affects residual stress profiles. In this study, the profiles of bead welded specimens were experimentally and analytically examined. The material used in this study was the low alloy steel SQV2A (JIS G 3120), which is equivalent to ASTM A533 Type B Class 1. Phase transformation usually occurs around the welding area in SQV2A and also affects the residual stress profiles. Two specimens were fabricated. One was single pass bead welded (single pass specimen) and the other was five pass bead welded (five pass specimen). Residual stresses of these specimens were measured by using the strain relief method. The measurement results show that longitudinal residual stress on the welded surface of the single pass specimen was lower than that of the opposite side surface due to phase transformation. Longitudinal residual stress around the 5th pass area of the five pass specimen was similar to that around the 1st pass area in the single pass specimen. The residual stress in the previous passes area, where passes from the 1st to the 4th were welded, was higher than that of the 5th pass area. These findings show that residual stress occurred around the welding area of these specimens and that the subsequent weld passes affect the profiles. To estimate the residual stress profiles numerically, temperature history analysis and residual stress analysis were conducted. Phase transformation strain was taken into consideration in these analyses as a component of thermal strain. The analysis results are consistent with the experiment results.