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2019 Vol.37, Issue 1 Preview Page

Research Article

Fruit Ripening and Related Gene Expression in ‘Goldone’ and ‘Jecy Gold’ Kiwifruit by Exogenous Ethylene Application

‘골드원’, ‘제시골드’ 키위프루트 과실의 에틸렌 처리에 따른 과실 후숙 및 관련 유전자의 발현

Mi Hee Shin1
,
Yong-Bum Kwack2
,
Yun-Hee Kim34
,
Jin Gook Kim14*
신 미희1
,
곽 용범2
,
김 윤희34
,
김 진국14*
1Department of Horticulture, Gyeongsang National University
2Namhae Sub-Station, National Institute of Horticultural and Herbal Science, Rural Development Administration, Namhae
3Department of Biology Education, Gyeongsang National University
4Institute of Agriculture and Life Science, Gyeongsang National University
1경상대학교 농업생명과학대학 원예학과
2농촌진흥청 국립원예특작과학원 남해출장소
3경상대학교 사범대학 생물교육과
4경상대학교 농업생명과학연구원
* Corresponding Author. 
28 February 2019. pp. 54-64
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Abstract

We studied the effect of exogenous ethylene application on fruit ripening and its related gene expression in kiwifruit (Actinidia chinensis) ‘Goldone’ and ‘Jecy Gold’ grown in Korea. The fruit ripening characteristics that were affected by exogenous ethylene were evaluated to implement the best post-harvest management practices for these two cultivars. Exogenous ethylene was applied in three different concentrations, and gene expression related to cell wall degradation, ethylene biosynthesis, and fruit quality changes were examined. The kiwifruits treated with exogenous ethylene ripened very fast compared to non-treated kiwifruits. A. chinensis ‘Jecy Gold’ was relatively insensitive to exogenous ethylene treatment when compared to A. chinensis ‘Goldone’. Exogenous ethylene accelerated ethylene biosynthesis and signaling pathways through changes in the expression of ACO and ERF5 genes. Furthermore, PGC and EXP2 gene expression was affected by exogenous ethylene application, suggesting changes in cell wall degradation in response to ethylene signaling. Relatively high gene expression was identified in kiwifruits treated with exogenous ethylene compared to untreated kiwifruits. The distinct expression differences of the four genes, ACO, ERF5, PGC, and EXP2 were recorded in A. chinensis ‘Goldone’ by the concentration of exogenous ethylene and days after treatment.

Keywords
acidity
ethephon
firmness
fruit quality
soluble solids content

본 연구는 외생 에틸렌 처리에 따른 키위프루트 과실의 후숙 특성을 이해하고, 국내 육성 품종에 대한 적절한 수확후 관리 방법을 구명하고자 실시하였다. 국내 육성품종인 ‘골드원’과 ‘제시골드’ 두 품종을 대상으로 외생 에틸렌 0, 10, 100µL·L-1의 세 가지 농도로 처리하여 과실의 품질 변화, 세포벽 분해 및 에틸렌 생합성과 관련된 유전자 발현 양상을 조사하였다. 외생 에틸렌 처리에 따른 과실의 품질 변화는 두 품종 모두 외생 에틸렌 처리 과실이 무처리 과실보다 빠르게 변화하였고, 높은 처리농도에서 더욱 빠르게 변화하였지만 ‘제시골드’ 품종이 ‘골드원’ 품종과 비교하여 상대적으로 외생 에틸렌 처리에 둔감한 반응을 보였다. 과실의 유전자 발현은 ACO, ERF5, PGC, EXP2 4가지 유전자 발현이 확인되었다. 4가지 유전자 모두 ‘제시골드’보다 ‘골드원’ 품종에서 분명한 발현 차이를 보였고, 외생 에틸렌 처리농도와 처리시간에 따라 높은 발현을 보였다.

키워드
산도
에테폰
경도
과실 품질
가용성 고형물 함량
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 37
  • No :1
  • Pages :54-64
  • Received Date : 2018-05-21
  • Revised Date : 2018-10-01
  • Accepted Date : 2018-09-18
  • DOI :https://doi.org/10.12972/kjhst.20190006
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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