Experiments were carried out to determine whether Japancse Lymnaea species could serve as the intermediate host of Australian Fasciola hepatica. The infection rate in the Lymnaea species exposed to miracidia were 83.3% in L. ollula and 38.5% in L. auricularia swinhoei, while L. truncatula and L. japonica were not infected. In L. ollula the fluke larvae developed rapidly; and the first emergence of cercariae was observed between 26 to 44 days (average, 31.8) after exposure and the total average number of metacercariae produced was 781.9 per snail. In L. a. swinhoei the flukes developed up to the redial stage, which contained early stages of cercariae. Prepatent period was 49 days in male 8-week-old Wistar rats inoculated with 20 metacercariae, and adult flukes recovered from the rats were morphologically characteristic of F. hepatica. These results suggest the possible establishment of Australian F. hepatica in Japan since L. ollula is a suitable intermediate host of the parasite.