Abnormal clonalities of B-lymphocytes in bovine leukemia virus-infected          cattle with persistent lymphocytosis

Shiho TAKEZAWA; Masaki MAEZAWA; Satoko TSUZUKU; Junko KAWAKAMI; Yoshinao OOUCI; Hisashi INOKUMA

doi:10.1292/jvms.21-0357

Internal Medicine

Abnormal clonalities of B-lymphocytes in bovine leukemia virus-infected cattle with persistent lymphocytosis

Shiho TAKEZAWA, Masaki MAEZAWA, Satoko TSUZUKU, Junko KAWAKAMI, Yoshinao OOUCI, Hisashi INOKUMA

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Keywords: bovine leukemia virus, minor-clonal proliferation, oligoclonal proliferation, persistent lymphocytosis, prognosis factor

JOURNAL OPEN ACCESS

2021 Volume 83 Issue 12 Pages 1928-1932

DOI https://doi.org/10.1292/jvms.21-0357

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Abstract

Peripheral B-lymphocyte clonality of 274 bovine leukemia virus-infected cattle with lymphocytosis was analyzed using clonality PCR based on sequences of the variable region of the bovine immunoglobulin H chain. None of the cattle showed monoclonal proliferation, while 10, 31, and 233 showed minor-clonal, oligoclonal, and polyclonal proliferation, respectively. A total of 163 cattle were analyzable the following year, and lymphocytosis was maintained in 157, indicating persistent lymphocytosis (PL). B-lymphocyte clonality of the 157 PL cattle was minor-clonal in 6 (3.8%), oligoclonal in 8 (5.1%), and polyclonal in 143 (91.1%). A higher rate of enzootic bovine leukosis (EBL) onset within a year was observed in PL cattle with minor-clonal (50.0% (3/6)) and oligoclonal (25.0% (2/8)) proliferation compared to those with polyclonal (5.6% (8/143)) proliferation. Minor-clonal and oligoclonal proliferation in PL cattle may be a prognosis factor for developing EBL.

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