Antimicrobial activity of tea catechin against canine oral bacteria and the          functional mechanisms

Lanlan BAI; Shiaki TAKAGI; Tasuke ANDO; Hiroshi YONEYAMA; Kumiko ITO; Hiroyuki MIZUGAI; Emiko ISOGAI

doi:10.1292/jvms.16-0198

Bacteriology

Antimicrobial activity of tea catechin against canine oral bacteria and the functional mechanisms

Lanlan BAI, Shiaki TAKAGI, Tasuke ANDO, Hiroshi YONEYAMA, Kumiko ITO, Hiroyuki MIZUGAI, Emiko ISOGAI

Author information

Lanlan BAI
Laboratory of Animal Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1–1 Tsutsumidori, Amamiya-machi Aoba-ku, Sendai, Miyagi 981–8555, Japan
Shiaki TAKAGI
Laboratory of Animal Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1–1 Tsutsumidori, Amamiya-machi Aoba-ku, Sendai, Miyagi 981–8555, Japan
Tasuke ANDO
Laboratory of Animal Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1–1 Tsutsumidori, Amamiya-machi Aoba-ku, Sendai, Miyagi 981–8555, Japan
Hiroshi YONEYAMA
Laboratory of Animal Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1–1 Tsutsumidori, Amamiya-machi Aoba-ku, Sendai, Miyagi 981–8555, Japan
Kumiko ITO
Electron Microscope room, Graduate School of Agricultural Science, Tohoku University, 1–1 Tsutsumidori, Amamiya-machi Aoba-ku, Sendai, Miyagi 981–8555, Japan
Hiroyuki MIZUGAI
Department of Preventive Dentistry, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Tobetsu, Ishikari-gun, Hokkaido 061–0293, Japan
Emiko ISOGAI
Laboratory of Animal Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1–1 Tsutsumidori, Amamiya-machi Aoba-ku, Sendai, Miyagi 981–8555, Japan

Keywords: biofilm, EGCG, polyphenolic compounds, streptococcal lipoteichoic acid

JOURNAL FREE ACCESS

2016 Volume 78 Issue 9 Pages 1439-1445

DOI https://doi.org/10.1292/jvms.16-0198

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Abstract

Epigallocatechin gallate (EGCG) is the major polyphenolic compound of green tea. Polyphenolic compounds were extracted from the leaf of Camellia sinensis (Japanese green tea), and the minimum inhibitory concentration against canine oral bacteria was measured. Subsequently, we investigated the inhibitory effects of polyphenolic compounds and EGCG on the growth of canine oral bacteria. EGCG showed antimicrobial activity against a model bacterium, Streptococcus mutans. Our results indicate that EGCG can inhibit the growth and biofilm formation of S. mutans and that EGCG does not interact with streptococcal lipoteichoic acid (LTA). Furthermore, our findings suggest that EGCG interacts with other component(s) of the bacterial membrane aside from streptococcal LTA to inhibit biofilm formation and damage biofilms.

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