Effect of Pre-harvest Irradiation of UV-A and UV-B LED in Ginsenosides Content of Ginseng Sprouts

Seong-Nam Jang; Ga-Oun Lee; Han-Sol Sim; Jin-Su Bae; Ae-Ryeon Lee; Du-Yong Cho; Kye-Man Cho; Ki-Ho Son

doi:10.12791/KSBEC.2022.31.1.028

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2022 Vol.31, Issue 1 Preview Page Next Page

Original Articles

Effect of Pre-harvest Irradiation of UV-A and UV-B LED in Ginsenosides Content of Ginseng Sprouts 새싹 인삼의 수확 전 UV-A 및 -B LED의 조사에 의한 진세노사이드의 영향

31 January 2022. pp. 28-34

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Abstract

This study was conducted to determine the changes in ginsenosides content according to additional UV-A, and UV-B LED irradiation before harvesting the ginseng sprouts. One-year-old ginseng seedlings (n=100) were transplanted in a tray containing a ginseng medium. The ginseng sprouts were grown for 37 days at a temperature of 20°C (24h), a humidity of 70%, and an average light intensity of 80 µmol·m^-2·s^-1 (photoperiod; 24h) in a container-type plant factory. Ginseng sprouts were then transferred to a custom chamber equipped with UV-A (370 nm; 12.90 W·m^-2) and UV-B (300 nm; 0.31 W·m^-2) LEDs and treated for 3 days. Growth parameters and ginsenoside contents in shoot and root were conducted by harvesting on days 0 (control), 1, 2, and 3 of UV treatments, respectively. The growth parameters showed non-significant differences between the control and the UV treatments (wavelengths or the number of days). Ginsenoside contents of the shoot was highly improved by 186% in UV-A treatment compared to the control in 3 days of the treatment time. The ginsenoside contents of the roots was more improved in UV-A 1-day treatment and UV-B 3-day treatment, compared to the control by 171% and 160%, respectively. As a result of this experiment, it is thought that UV LED irradiation before harvesting can produce sprout ginseng with high ginsenoside contents in a plant factory.

Keywords

ginseng

ginsenoside

medicinal plant

plant factory

본 연구는 새싹 인삼의 수확 전에 UV-A, UV-B LED의 추가 조사에 따른 ginsenoside 함량 변화를 알아보기 위해 수행되었다. 실험 재료는 1년근 묘삼을 사용하였고, 인삼 전용 상토를 채운 삽목상자에 100개체를 정식하였다. 그 후, 묘삼을 온도 20°C, 습도 70%, 평균 광도 80µmol·m^-2·s^-1(광주기; 24h)로 유지되는 컨테이너형 식물 공장에서 37일 동안 재배하였다. 37일 동안 재배한 새싹 인삼을 UV-A(370nm; 12.90W·m^-2) 및 UV-B(300nm; 0.31W·m^-2) LED가 장착된 맞춤형 챔버로 옮겨 3일 동안 처리하였다. 생육조사 및 ginsenoside 함량 분석은 UV 처리 0일(대조구), 1일, 2일 및 3일에 각각 수확하여 수행하였다. 그 결과, 생육은 대조구와 UV 파장 및 일수에 따른 통계적 차이를 보이지 않았다. 반면에 UV-A 처리 3일 만에 지상부의 ginsenoside 함량이 대조구에 비해 186% 향상되었다. 뿌리의 ginsenoside 함량은 UV-A 1일 처리 및 UV-B 3일 처리에서 대조구에 비해 각각 171% 및 160% 더 증가하였다. 본 실험의 결과, 식물공장에서 수확 전 UV LED 조사를 통해 ginsenoside 함량이 높은 새싹 인삼을 생산할 수 있는 것으로 사료된다.

키워드

식물공장

약용식물

인삼

진세노사이드

References

Cha M.K., J.H. Cho, and Y.Y. Cho 2013, Growth of leaf lettuce as affected by light quality of LED in closed-type plant factory system. Protected Hort Plant Fac 22:291-297. (in Korean) doi:10.12791/KSBEC.2013.22.4.291 10.12791/KSBEC.2013.22.4.291

Chae S, K.A. Kang, W.Y. Chang, M.J. Kim, S.J. Lee, Y.S. Lee, H.S. Kim, D.H. Kim, and J.W. Hyun 2009, Effect of compound K, a metabolite of ginseng saponin, combined with γ-Ray radiation in human lung cancer cells in vitro and in vivo. J Agric and Food Chem 57:5777-5782. doi:10.1021/jf900331g 10.1021/jf900331g19526988

Choi J.E., X. Li, Y.H. Han, and K.T. Lee 2009, Changes of saponin contents of leaves, stems and flower-buds of Panax ginseng C. A. Meyer by harvesting days. Korean J Medicinal Crop Sci 17:251-256. (in Korean)

Choi S.Y., C.W. Cho, Y. Lee, S.S. Kim, S.H. Lee, and K.T. Kim 2012, Comparison of ginsenoside and phenolic ingredient contents in hydroponically-cultivated ginseng leaves, fruits, and roots. J Ginseng Res 36:425-429. doi:10.5142/jgr.2012.36.4.425 10.5142/jgr.2012.36.4.42523717146PMC3659603

Gartia S., M.K. Pradhan, P.N. Joshi, U.C. Biswal, and B. Biswal 2003, UV-A irradiation guards the photosynthetic apparatus against UV-B- induced damage. Photosynthetica 41:545-549. doi:10.1023/B:PHOT.0000027518.95032.c1 10.1023/B:PHOT.0000027518.95032.c1

Han J.Y., M.J. Kim, Y.W. Ban, H.S. Hwang and Y.E. Choi 2013, The involvement of β-amyrin 28-oxidase (CYP716 A52v2) in oleanane-type ginsenoside biosynthesis in Panax ginseng. Plant Cell Physiol 54:2034-2046. doi:10.1093/pcp/pct141 10.1093/pcp/pct14124092881

Harkey M.R., G.L. Henderson, M.E. Gershwin, J.S. Stern, and R.M. Hackman 2001, Variability in commercial ginseng products: an analysis of 25 preparations. Am J Clin Nutr 73:1101-1106. doi:10.1093/ajcn/73.6.1101. 10.1093/ajcn/73.6.110111382666

Hwang I.G., H.Y. Kim, E.M. Joung, K.S. Woo, J.H. Jeong, K.W. Yu, J Lee, and H.S. Jeong 2010, Changes in ginsenosides and antioxidant activity of Korean ginseng (Panax ginseng C.A. Meyer) with heating temperature and pressure. Food Sci Biotechnol 19:941-949. doi10.1007/s10068-010-0132-9 10.1007/s10068-010-0132-9

In J.G., D.S. Park, B.S. Lee, T.H. Lee, S.Y. Kim, Y.D. Rho, D.H. Cho, C.W. Jin, and D.C. Yang 2006, Effects of white light and UV irradiation on growth and saponin production from ginseng hairy root. Korean J Medicinal Crop Sci 14:360-366. (in Korean)

Jang I., G. Do, S. Suh, J. Yu, I. Jang, J. Moon, and C. Chun 2020, Physiological responses and ginsenoside production of Panax ginseng seedlings grown under various ratios of red to blue light-emitting diodes. Hortic Environ Biotechnol 61:663-672. doi:10.1007/s13580-020-00255-5 10.1007/s13580-020-00255-5

Jang I.B., J. Yu, S.J. Suh, I.B. Jang, and K.B. Kwon 2018, Growth and ginsenoside content in different parts of ginseng sprouts depending on harvest time. Korean J Medicinal Sci 26:205-213. 10.7783/KJMCS.2018.26.3.205

Jeong D.H., D.Y. Lee, I.B. Jang, J. Yu, K.C. Park, E.H. Lee, Y.J. Kim, and H.W. Park 2018, Growth and ginsenoside content of one year old ginseng seedlings in hydroponic culture over a range of days after transplanting. Korean J Medicinal Crop Sci 26:464-470. (in Korean) doi:10.7783/kjmcs.2018.26.6.464 10.7783/KJMCS.2018.26.6.464

Jin Y., Y.J. Kim, J.N. Jeon, C. Wang, J.W. Min, S.Y. Jung, and D.C. Yang 2012, Changes of ginsenosides and physiochemical properties in ginseng by new 9 repetitive steaming and drying process. Korean J Plant Res 25:473-481. (in Korean) doi:10.7732/KJPR.2012.25.4.473. 10.7732/kjpr.2012.25.4.473

Jung M.Y., B.S. Jeon, and J.Y. Bock 2002, Free, esterified, and insoluble-bound phenolic acids in white and red Korean ginsengs (Panax ginseng C.A. Meyer). Food Chem 79:105-111. doi:10.1016/S0308-8146(02)00185-1 10.1016/S0308-8146(02)00185-1

Kim J.H., Y.S. Yi, M.Y. Kim, and J.Y. Cho 2017, Role of ginsenosides, the main active components of Panax ginseng, in inflammatory responses and diseases. J Ginseng Res 41:435-443. doi:10.1016/j.jgr.2016.08.004 10.1016/j.jgr.2016.08.00429021688PMC5628327

Kim Y.J., T.K.L. Nguyen, and M.M. Oh 2020, Growth and ginsenosides content of ginseng sprouts according to LED-based light quality changes. Agronomy 10:1979. doi:10.3390/agronomy10121979 10.3390/agronomy10121979

Kozai T. 2013, Resource use efficiency of closed plant production system with artificial light: Concept, estimation and application to plant factory. Proc Jpn Acad Ser B Phys Biol Sci 89:447-461. doi:10.2183/pjab.89.447 10.2183/pjab.89.44724334509PMC3881955

Lee J.H., M.M. Oh, and K.H. Son 2019, Short-term ultraviolet (UV)-a light-emitting diode (LED) radiation improves biomass and bioactive compounds of kale. Front Plant Sci 10:1042. doi:10.3389/fpls.2019.01042 10.3389/fpls.2019.0104231481968PMC6710713

Li N., B. Liu, D.E. Dluzen, and Y. Jin 2007, Protective effects of ginsenoside Rg2 against glutamate-induced neurotoxicity in PC12 cells. J Ethnopharmacol 111:458-463. doi:10.1016/j.jep.2006.12.015 10.1016/j.jep.2006.12.01517257792

Park E.K., M.K. Choo, M.J. Han, and D.H. Kim 2004, Ginsenoside Rh1 possesses antiallergic and anti-inflammatory activities. Int Arch Allergy Immunol 133:113-120. doi:10.1159/000076383 10.1159/00007638314739579

Park S.M., E.H. Jung, J.K. Kim, K.H. Jegal, C.A. Park, I.J. Cho, and S.C. Kim 2017, 20S-Protopanaxadiol, and aglycosylated ginsenoside metabolite, induces hepatic stellate cell apoptosis through liver kinase B1-AMP-activated protein kinase activation. J Ginseng Res 41:392-402. doi:10.1016/j.jgr.2017.01.012 10.1016/j.jgr.2017.01.01228701883PMC5489770

Yang H.C. 1977, Studies on the saponin of ginseng leaves. Research Paper in Chungnam University 8:117-121.

Yu H., Q. Liu, C. Zhang, M. Lu, Y. Fu, W.T. Im, S.T. Lee, and F. Jin 2009, A new ginsenosidase from Aspergillus strain hydrolyzing 20-O-multi-glycoside of PPD ginsenoside. Process Biochem 44: 772-775. doi:10.1016/J.PROCBIO.2009.02.005 10.1016/j.procbio.2009.02.005

Information

Publisher :The Korean Society for Bio-Environment Control
Publisher(Ko) :(사)한국생물환경조절학회
Journal Title :Journal of Bio-Environment Control
Journal Title(Ko) :생물환경조절학회지
Volume : 31
No :1
Pages :28-34
Received Date : 2021-12-29
Revised Date : 2022-01-10
Accepted Date : 2022-01-12
DOI :https://doi.org/10.12791/KSBEC.2022.31.1.028

Journal of Bio-Environment Control ISSN:1229-4675(Print) 2765-3641(Online) 생물환경조절학회지

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