The steam volatile constituents of wild Wasabi and Orandagarashi have been studied by combination of chromatographic and spectroscopic methods. Each oil was separated into hexane, benzene, ether and methanol eluted fractions by column chromatography. As a result of column chromatography, sulfur and nitrogen constituents were eluted with benzene and ether.
14 sulfur and nitrogen compounds in the steam volatile oil of Wasabia japonica and 7 sulfur and nitrogen compounds in the steam volatile oil of Nasturitium officinale were detected.
The characteristic major components of Wasabia japonica were allyl isothiocyanate, 6-methylthiohexanonitrile, 7-methylthioheptanonitrile and 6-methylthiohexyl isothiocyanate, and those of Nasturitium officinale were 3-phenylpropiononitrile, 8-methylthiooctanonitrile and 9-methylthiononanonitrile.