Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Intracellular Distribution and Some Properties of β-Glucosidases of a Cellulolytic Pseudomonad
Jun-Ton HWANGHiroshi SUZUKI
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1976 Volume 40 Issue 11 Pages 2169-2175

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Abstract

Two distinct forms of β-glucosidase, A and B, were found to occur in the cells of Pseudomonasfluorescens var. cellulosa: A was membrane-bound, while B cytosolic. They differed also from each other in some properties, such as molecular size, kinetic parameters, and susceptibility to various compounds. β-Glucosidase B was partially purified and studied especially of its substrate specificity. The results indicated that it may be an atypical β-glucosidase which possesses a certain character of exo-cellulase.

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