Waxy wheat cultivar with no amylose in endosperm starch has never been reported. We tried to develop waxy wheat cultivar based on a combination of partial waxy mutants lacking one and two of the three proteins by haploid method of breeding in order to raise fixed lines in a short period. We used two common wheats, Kanto 107' Iacking both the Wx-AI and Wx-BI proteins, and Bai Huo, lack-ing the Wx-DI protein. Out of 19 doubled haploid (DH) plants, 2 plants were identified as waxy genotype by iodine solution reaction and amylose content. Electrophoretic analysis did not detect the Wx protein in these waxy phenotypes. The x2 test among seeds (, glassy + fnoury seeds) collected at random for inheritance of the waxy character at F₂ generation, indicated that the observed frequency was not significantly different from the expected 63:1 seregation ratio. In 1000 floury F₂ seeds, however, the x2 test indicated that this frequency was significantly different from the expected 63:1 segregation ratio. Namely, waxy seeds were observed more than the expected segregation ratio in floury F₂ seeds. One (C-D 1478) of two waxy wheats was similar to the male parent, 'Bai Huo' except the culm length, while the other (C-D 1479) was similar to the female parent, 'Kanto 107'.