1995 Volume 45 Issue 4 Pages 413-417
Suspension cultures of calli derived from hypocotyl explant of Morus alba L. var. MR2 have produced embryo like structures. Calli were induced on the semisolid MS medium supplemented with 1.0 mg/l 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 0.5 mg/l 6-benzylaminopurine (BAP). They were transferred to MS Iiquid medium with different concentrations (0.1-1.0 mg/l ) of 2, 4-D and further subcultured at weekly intervals into fresh medium. Embryo-like structures were observed at each stage of development. Cells in the suspension culture having enriched cytoplasm with prominent nuclei, divided to form filamentous structures and continuous division of these structures lead to the development of globular and heartshaped structures. Suspension medium containing 0.5 mg/l 2, 4-D was essen-tial to induce high frequencies of embryo like structures while lower (0.1-0.2 mg/l ) and higher (1.0 mg/l ) concentrations caused either root or callus formation respectivelv. . There was no further development of the heartshaped