In this paper we described the successful development of kiwi fruit transformants from hairy roots induced by A. rhizogenes. Hypocotyls of kiwi fruit seedlings inoculated with a domestic wild strain of A. rhizogenes NIAES 1724 produced two hairy root lines growing vigorously on MS medium without phytohormones. Shoots were easily differentiated from the hairy roots, and gave rise to complete plants. These plants developed shorter internodes, darker wrinkled leaves and displayed active root formation under in vitro conditions. The number of branches increased and miniaturization of leaves was promoted after acclimatization, as compared with the control plants. They were found to be transformants because of the detection of mikimopine in the leaf extracts and the presence of rol genes from Ri plasmid in the plant total DNA. These results suggest that A. rhizogenes NIAES 1724 could be utilized as a vector for transformation studies of kiwi fruit.