1991 Volume 41 Issue 1 Pages 169-173
Embryogenic and nonembryogenic calli of carrot were visually selected and cultured individually for 3 weeks on MURASHIC.E and SKOOG'S (MS) agar medium supplemented with 5 x 10-6 M 2, 4-dichlorophenoxyacetic acid (2, 4-D). They were dried to a moisture level of about 3% for 6 hours and recultured on MS medium without 2, 4-D. All the dried embryogenic calli survived and formed many embryos or plantlets. The dried nonembryogenic calli could not survive except for very small parts which appeared to be embryogenic calli. These results showed the possibility of selection of embryogenic calli by the application of the drying method.