Maintenance of valuable genotypes of common buckwheat is difiicult owing to heterostylic self-incompatibility. To establish vegetative propagation system, a procedure for plant regeneration from immature inflorescence culture was developed. Immature inflorescences of common buckwheat and perennial buckwheat were cultured on B5 media supplemented with NAA, 2, 4-D and BA at various concentrations. Direct shoot production from the inflorescence of common buckwheat was promoted by the addition of 0-2.0 mg/l NAA+0-2.0mg/l BA (optimum. 0.2 mg/l NAA+1. 0 mg/l BA). In perennial buckwheat, direct shoot was induced only on the medium containing 1.0mg/l NAA and l.0mg/l BA with lower frequency. than common buckwheat. In the presence of more than 1.0mg/l 2, 4-D or more than 2.0mg/l NAA, callus was well formed in both species. Shoots were induced from common. buckwheat callus on B5 medium containing 0.2 mg/l NAA and 1.0mg/l BA and lacking plant growth regulators, but not from perennial buckwheat. The shoots obtained by the two different pathways developed a root system on MS medium containing 1.0 mg/l IBA. Chromosomal analysis of the directly induced regenerants showed that they had diploid chromosome numbers (2 n=16).