From the tissue cultures of syncarp, slip, crown and axillary bud of Ananas comosus (L.) Merr., cultivar Smooth Cayenne, 448 plants were established. To exarnine the two possible merits of this technique on self-incompatible plants of vegetative propagation, mass-production of non-variants and induction of variants useful in breeding, the variations among the redifferentiated plants were investigated. Many variants regarding spine, leaf color, wax secretion on leaf surface and foliage density were found among the established plants. There were a few variarlts with narrow leaves or albino stripes. Organ-to-organ differences were found in the fre-quencies and the modes of distribution of these variations. Syncarps and a slip developed variants in high frequencies, while crowns and axillary buds did only in low frequencies. The variants from syncarps included the characters on leaf color, spine, wax and foliage density, but the ones from a slip, crowns and axillary bu.ds were almost confined in spine characters. From these present findings, it is considered possible to apply the tissue culture technique on the rapid propagation of non-variant plantlets and the induction of useful variants, if proper kinds of organs are chosen for the respective purpose.