A specific relationship between the nuclear genes and the genetic factors of cytoplasms as a consequence of cytoplasmic mutation was confirmed from the genetic interaction between the pollen fertility restoring genes and the sterile cytoplasms which were induced by gamma irradiation from the normal (N) cytoplasm, The two complementary genes Rf₄ and Rf₅ responsible for S₁_-₂ cytoplasm were different from those for S cytoplasm of spontaneous origin, and F₃ lines having a genotype S₁_-₂ Rf₄Rf₄Rf₅Rf₅ Were bred true to the pollen fertility restored types, N or S.S.a. In the pollen fertility restoration for S₁_-₃ cytoplasm, an epistatic relation of two alleles (Rf₆ and Rf₇) which are different from S and S₁_-₂ showed various segregation ratios depend-ing on parental genotypes. Although the segregation mode of two additive genes was similar to those in S cytoplasm, the new genes Rf₈ and Rfg were responsible fcr S₁_-₄ cytoplasm. Thus there is the specific relation between the pollen fertility restoring genes and the genetic factors of the male sterile cytoplasms. It is probable that the cytoplasmic mutations occurred in the strain H-2002 which contains normal (N) cytoplasm and diverse genotypes on pollen restoration and then the combination between the altered cytoplasm and the recessive type of the specific pollen fertility restoring genes indicated male sterility. If the maintainer and fcrtility restorer lines for the induced cytoplasms were selected based on the inheritance mode of pollen fertility restoration, they are also available for the use of the male sterility in hybrid seed production.