DETECTION OF GANGLIOSIDE GM1 IN PRIMARY CULTURED CELLS OF RAT BRAIN AVIDIN-BIOTIN-CHOLERAGEN AND AVIDIN-BIOTIN ANTIBODY COMPLEX STAINING METHODS

HIROAKI ASOU; NORIKO IWASAKI-MUTOU; SHUSUKE HIRANO; TAKASHI TURUMIZU; YOSHIMUNE HORIBE

doi:10.1267/ahc.18.383

DETECTION OF GANGLIOSIDE GM1 IN PRIMARY CULTURED CELLS OF RAT BRAIN

AVIDIN-BIOTIN-CHOLERAGEN AND AVIDIN-BIOTIN ANTIBODY COMPLEX STAINING METHODS

HIROAKI ASOU, NORIKO IWASAKI-MUTOU, SHUSUKE HIRANO, TAKASHI TURUMIZU, YOSHIMUNE HORIBE

Author information

JOURNAL FREE ACCESS

1985 Volume 18 Issue 4 Pages 383-389

DOI https://doi.org/10.1267/ahc.18.383

Details

Abstract

A new technique demonstrating the presence of ganglioside GM1 in neurons, neurite bundles and oligodendrocytes using avidin-biotin complex systems in double staining techniques is reported. The method was applied to cultured brain cells.
The ganglioside GM1 may be present in the growth cones of outgrowing neurites in these living cell cultures.

Corresponding author

Register with J-STAGE for free!