A method for the determination of glycyrrhizin in liquorice roots and its preparation was devised by the use of a high-speed liquid chromatography. Separation can be achieved within 12 min employing a 1-m column packed with Permaphase AAX pellicular anion exchanger, using gradient from H₂O to 0.05M NaClO₄ solution as an eluant in a Du Pont LC 830 liquid chromatograph. Accuracy in this method was found to be less than ±1.5%, with the relation Y=0.856X-0.538, and a relative coefficient of 0.999.