Separatory determination of alkaloids was carried out on 10 kinds of extract of plants containing berberine-type alkaloids and comparative examinations were made on the action of each alkaloid on mouse uterus and intestine, antibacterial activity, and acute toxicity (LD₅₀), observing individuality and similarity among these plants. Content of berberine was the maximum in Coptis japonica and that of the total crude tertiary bases was the greatest in Hydrastis canadensis. All the extracts had anticonvulsive action on intestines and uterus, except Jateorrhiza palmata and Coptis japonica which contracted the uterus. The total crude tertiary base also had anticonvulsive action. Berberine, palmatine, jatrorrhizine, several of tertiary bases, and the extract of Coptics japonica were effective against Staphylococcus aureus 209P in 1000-6000 dilution, berberine being the strogest. Acute toxicity (LD₅₀) was the maximum in Coptis japonica, and lowest in Nandina domestica and Berberis Thunbergii. In evaluating the physiological action of these plants, the alkaloids contained in them have little specificity for comparing these plants as containing such alkaloids. It seems necessary to consider the specificity of each individual plant.