From the leaves of Phellodendron japonicum MAXIM. (Rutaceae), six new flavonoid glycosides (I-VI) were isolated, together with eight known compounds. The structures of I-VI were shown to be 8-prenyl-3, 4', 5-trihydroxy-flavone 7-O-β-D-(6-O-malonyl) glucopyranoside, (2R, 3R)-8-prenyl-3, 4', 5-trihydroxyflavanone 7-O-β-D-(6-O-malonyl) glucopyranoside, 8-[(S)-2, 3-dihydroxy-3-methylbutyl]-3, 4', 5-trihydroxyflavone 7-O-β-D-glucopyranoside, 8-[(R)-2, 3-dihydroxy-3-methylbutyl]-3, 4', 5-trihydroxyflavone 7-O-β-D-glucopyranoside, (2R, 3R)-8-[(S)-2, 3-dihydroxy-3-methylbutyl]-3, 4', 5-trihydroxyflavanone 7-O-β-D-glucopyranoside and (2R, 3R)-8-[(R)-2, 3-dihydroxy-3-methylbutyl]-3, 4', 5-trihydroxyflavanone 7-O-β-D-glucopyranoside, respectively, on the basis of the chemical and spectral data.