This paper describes high performance liquid chromatographic (HPLC) determinations of halogenated pyrenes in water by use of LiChrosorb RP-18 column as a stationary phase and acetonitrile/water as a mobile phase. The method consists of the following 3 procedures : extraction of halogenated pyrenes from water samples with benzene, cleaning up by one dimensional dual-band thin layer chromatography, identification and determination of halogenated pyrenes by HPLC. The calibration curves of pyrene (Py), 1-chloropyrene (PyCl), 1-bromopyrene (PyBr), 1, 8-, 1, 6-, 1, 3-dichloropyrene (PyCl2) and 1, 8-dibromopyrene (PyBr2) were linear over the range of 0-2 ng for Py and PyCl, 0-4 ng for each PyCl2, and 0-20 ng for PyBr and 1, 8-PyBr2. The minimum detectable amounts of Py and each halogenated pyrenes (s/n=4) were 0.1 ng for Py, 0.05 ng for PyCl, 0.2 ng for 1, 8-PyCl2, 0.25 ng for 1, 6-PyCl2, 0.7 ng for 1, 3-PyCl2 and 1.0 ng for PyBr and PyBr2. The proposed method has been applied to determine the halogenated compounds in chlorinated-river water spiked with 100 μg/l pyrene. The concentrations of halogenated compounds were 0.67 μg/l for PyCl, 17.1 μg/l for PyBr, 3.87 μg/l for PyCl2 and 14.0 μg/l for PyBr2.
