Color reactions of 1, 3, 5-trinitrobenzene and acetophenone in methanol and methanol-water mixtures under the Janovsky conditions were investigated by use of the stopped flow rapid scan spectrophotometric technics. The observations that the color changes in methanol were quite different from those in methanol-water mixtures of high water content were discussed in comparison with the absorption spectral features of the coloring matters (I-Na and II-Na). The color reaction pathways presented in these media were summarized.