银杏雌、雄植株花芽分化后期及开花期间生理代谢的比较

doi:10.12302/j.issn.1000-2006.202101032

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南京林业大学学报（自然科学版） ›› 2022, Vol. 46 ›› Issue (4): 59-67.doi: 10.12302/j.issn.1000-2006.202101032

所属专题：中国特有孑遗树种银杏雌雄株发育生理机制

• 专题报道Ⅱ：中国特有孑遗树种银杏雌雄株发育生理机制（执行主编　方升佐） • 上一篇下一篇

银杏雌、雄植株花芽分化后期及开花期间生理代谢的比较

狄晶晶(), 叶威, 吴琴霞, 冯凯, 曹栋, 李强, 陈颖()

南京林业大学生物与环境学院,南方现代林业协同创新中心,江苏南京 210037

收稿日期:2021-01-25 修回日期:2021-04-07 出版日期:2022-07-30 发布日期:2022-08-01
通讯作者: 陈颖
基金资助:
国家自然科学基金项目(31770674);国家重点研发计划(2017YFD0601301);江苏高校优势学科建设工程资助项目(PAPD)

Comparisons of physiological metabolism of female and male Ginkgo biloba trees during the late flower bud differentiation and flowering

DI Jingjing(), YE Wei, WU Qinxia, FENG Kai, CAO Dong, LI Qiang, CHEN Ying()

Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment,Nanjing Forestry University, Nanjing 210037, China

Received:2021-01-25 Revised:2021-04-07 Online:2022-07-30 Published:2022-08-01
Contact: CHEN Ying

摘要/Abstract

摘要：

【目的】研究银杏雌、雄株花芽分化后期及开花期间内含物和激素代谢的动态变化,为银杏雌、雄株的定向培育、授粉、性别鉴定提供参考。【方法】通过采集银杏雌、雄株花芽分化后期(11月25日—次年3月16日)、开花期(3月17日—4月7日)、开花后(4月8日—4月28日)3个阶段短枝果芽及开花后的叶片,测定其可溶性糖、可溶性蛋白、黄酮和内酯等内含物及激素含量。【结果】在花芽分化后期,银杏雄株普遍比雌株含有更多的可溶性糖和可溶性蛋白,其苯丙氨酸解氨酶(PAL)活性也更高;而在开花期,银杏雌株比雄株含有更多的可溶性糖、淀粉和可溶性蛋白,其PAL活性也更高,但此期间雄株的赤霉素(GA)和玉米素(ZT)含量远高于雌株。雌、雄植株芽期的过氧化物酶(POD)活性远高于开花期和花后期,而开花期间雌、雄株叶片都具有较高的ZT、GA、总黄酮和萜内酯水平。【结论】银杏雌、雄株在开花时间各节点的生理代谢水平出现不同步性,雄株的可溶性糖、可溶性蛋白、总黄酮和萜内酯的高点比雌株早2周左右。较高水平的GA和ZT、总黄酮和萜内酯可能是银杏雄株开花及雌株胚珠发育所必需的。

关键词: 银杏, 雌、雄植株, 花芽分化后期, 开花, 生理代谢

Abstract:

【Objective】 The study aimed to investigate the dynamic changes in hormones and physiological metabolism indicators such as carbohydrates and flavonoids to provide a reference for the directional cultivation, pollination and gender identification of male and female Ginkgo biloba trees. 【Method】 Short fruit buds and leaves were collected from ginkgo trees during three developmental phases, that is, late flower bud differentiation (November 25 of previous year to March 16 of next year), flowering (March 17 to April 7), and after flowering (April 8 to April 28) to analyze the changes in the levels of hormones, carbohydrates, soluble proteins, total flavonoids, lactones, and peroxidase (POD) as well as phenylalanine ammonia-lyase (PAL) enzyme activities in female and male ginkgo trees. 【Result】 During the late flower bud differentiation stage, male trees had higher soluble sugar and protein contents and PAL activity than female trees, whereas during the flowering period, female trees had comparatively higher concentrations of soluble sugar, starch, soluble protein and PAL activity. However, gibberellin (GA) and zeatin (ZT) levels were significantly higher in the male trees during the flowering period. The considerably higher POD activity was observed in the female and male trees during the late flower bud differentiation period than during the other two periods, while the higher levels of ZT, GA, flavonoids and terpene lactones were observed during the flowering period. 【Conclusion】 Male and female ginkgo trees exhibited unsynchronized physiological metabolisms at the flowering time, and the peaks of soluble sugar, soluble protein, flavonoid and terpenoid lactone levels in male trees were observed approximately two weeks earlier than those in the female trees. High levels of GA, ZT, flavonoids and terpene lactones may be necessary for flowering and ovule development in male and female ginkgo trees, respectively.

Key words: Ginkgo biloba, male and female trees, late flower bud differentiation, flowering, physiological metabolism

中图分类号:

S722
Q945

狄晶晶,叶威,吴琴霞,等. 银杏雌、雄植株花芽分化后期及开花期间生理代谢的比较[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 59-67.

DI Jingjing, YE Wei, WU Qinxia, FENG Kai, CAO Dong, LI Qiang, CHEN Ying. Comparisons of physiological metabolism of female and male Ginkgo biloba trees during the late flower bud differentiation and flowering[J].Journal of Nanjing Forestry University (Natural Science Edition), 2022, 46(4): 59-67.DOI: 10.12302/j.issn.1000-2006.202101032.

图/表 7

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参考文献 35

[1]	曹福亮. 中国银杏志[M]. 北京: 中国林业出版社, 2007.
	CAO F L. Ginkgo biloba in China[M]. Beijing: China Forestry Publishing House, 2007.
[2]	黄茜, 陈珂, 刘霁瑶, 等. SCAR分子标记和叶形分析对银杏性别鉴定的研究[J]. 西南大学学报(自然科学版), 2016, 38(3):62-69.
	HUANG Q, CHEN K, LIU J Y, et al. Sex identification of Ginkgo biloba L. by SCAR markers and leaf morphology[J]. J Southwest Univ (Nat Sci Ed), 2016, 38(3):62-69.DOI:10.13718/j.cnki.xdzk.2016.03.011.
[3]	陈珂, 刘霁瑶, 蒋园园, 等. 成年银杏雌雄株非花果期形态学差异性比较分析[J]. 西南大学学报(自然科学版), 2017, 39(7):17-24.
	CHEN K, LIU J Y, JIANG Y Y, et al. Analysis of morphological differences of the leaves of adult ginkgo trees in the non-flowering and non-fruiting period[J]. J Southwest Univ (Nat Sci Ed), 2017, 39(7):17-24.DOI:10.13718/j.cnki.xdzk.2017.07.003.
[4]	杨金华, 付庆云. 雌雄异株植物银杏特异蛋白初步分析[J]. 科技信息, 2012(31): 241,202.
	YANG J H, FU Q Y. Preliminary analysis of ginkgo-specific proteins in dioecious plants[J]. Sci Technol Inf, 2012(31): 241,202.DOI:10.3969/j.issn.1001-9960.2012.31.167.
[5]	蒋雪梅, 戚文华, 肖娟, 等. 盐胁迫下外源脯氨酸对银杏雌雄幼苗生理生化特性的影响[J]. 植物生理学报, 2013, 49(6):579-585.
	JIANG X M, QI W H, XIAO J, et al. Effects of exogenous proline on physiological and biochemical features of salt-stressed female and male Ginkgo biloba L. seedlings[J]. Plant Physiol J, 2013, 49(6):579-585.DOI:10.13592/j.cnki.ppj.2013.06.006.
[6]	蔡汝, 陶俊, 陈鹏. 银杏雌雄株叶片光合特性、蒸腾速率及产量的比较研究[J]. 落叶果树, 2000, 32(1):14-15.
	CAI R, TAO J, CHEN P. Comparative study on photosynthetic characteristics, transpiration rate and yield of ginkgo male and female plants[J]. Deciduous Fruits, 2000, 32(1):14-15. DOI:10.13855/j.cnki.lygs.2000.01.005.
[7]	HE M, SHI D W, WEI X D, et al. Gender-related differences in adaptability to drought stress in the dioecious tree Ginkgo biloba[J]. Acta Physiol Plant, 2016, 38(5):1-14.DOI:10.1007/s11738-016-2148-0.
[8]	朱远方. 基于转录组学的银杏差异表达基因研究[D]. 广州: 华南理工大学, 2019.
	ZHU Y F. Research on differentially expressed genes of ginkgo based on transcriptomics[D]. Guangzhou: South China University of Technology, 2019.
[9]	张晓文. 银杏雌雄花芽差异表达基因研究[D]. 泰安: 山东农业大学, 2014.
	ZHANG X W. Study on the differentially expressed genes in male and female flower buds of Ginkgo biloba L.[D]. Tai’an: Shandong Agricultural University, 2014.
[10]	温银元, 王玉国, 尹美强, 等. 银杏试管苗的雌雄鉴别与调控[J]. 核农学报, 2010, 24(1):31-35.
	WEN Y Y, WANG Y G, YIN M Q, et al. Sex identification and regulation of ginkgo plantlets in vitro cultured[J]. J Nucl Agric Sci, 2010, 24(1):31-35.
[11]	史继孔, 樊卫国, 文晓鹏. 银杏雌花芽形态分化的研究[J]. 园艺学报, 1998(1):33-36.
	SHI J K, FAN W G, WEN X P. Morphological differentiation of female flower buds of Ginkgo biloba[J]. Acta Hortic Sin, 1998(1):33-36.
[12]	王建红, 李广, 车少臣, 等. 北京地区银杏雌株花芽分化期间内源激素变化及其花芽分化发端时间的确定[J]. 安徽农业科学, 2020, 48(13):118-123.
	WANG J H, LI G, CHE S C, et al. Changes of endogenous hormones during female floral bud differentiation of Ginkgo biloba L. in Beijing region and determination of the initiation time[J]. J Anhui Agric Sci, 2020, 48(13):118-123.DOI:10.3969/j.issn.0517-6611.2020.13.033.
[13]	张万萍, 史继孔, 樊卫国, 等. 银杏雄花芽的形态分化[J]. 园艺学报, 2001, 28(3):255-258.
	ZHANG W P, SHI J K, FAN W G, et al. Morphological differentiation of male flower bud of Ginkgo biloba L.[J]. Acta Houticulturae Sin, 2001, 28(3):255-258.DOI:10.3321/j.issn:0513-353X.2001.03.013.
[14]	钟川, 王熊军, 漆小雪, 等. 银杏雌雄株花期内源激素和养分含量动态变化规律[J]. 江苏农业科学, 2011, 39(6):251-254.
	ZHONG C, WANG X J, QI X X, et al. Dynamic changes of endogenous hormone and nutrient content in female and male plants of Ginkgo biloba[J]. Jiangsu Agric Sci, 2011, 39(6):251-254.DOI:10.15889/j.issn.1002-1302.2011.06.237.
[15]	王学奎. 植物生理生化实验原理和技术[M]. 2版. 北京: 高等教育出版社, 2006.
	WANG X K. Principles and techniques of plant physiological biochemical experiment[M]. 2nd ed. Beijing: Higher Education Press, 2006.
[16]	CHEN Y, LIN F Z, YANG H, et al. Effect of varying NaCl doses on flavonoid production in suspension cells of Ginkgo biloba: relationship to chlorophyll fluorescence,ion homeostasis,antioxidant system and ultrastructure[J]. Acta Physiol Plant, 2014, 36(12):3173-3187.DOI:10.1007/s11738-014-1684-8.
[17]	邓桂春, 王鑫, 赵丽艳, 等. 分光光度法测定银杏叶中黄酮的含量[J]. 辽宁大学学报(自然科学版), 2005, 32(2):101-104.
	DENG G C, WANG X, ZHAO L Y, et al. Spectrophotometric determination of total flavonoids in Ginkgo leaves[J]. J Liaoning Univ (Nat Sci Ed), 2005, 32(2):101-104.DOI: 10.3969/j.issn.1000-5846.2005.02.002.
[18]	张渝阳, 王鑫, 赵丽娟, 等. 分光光度法测定银杏萜内酯的含量[J]. 辽宁化工, 2006, 35(10):618-620.
	ZHANG Y Y, WANG X, ZHAO L J, et al. Spectrophotometric determination of total terpenoids in ginkgo leaves[J]. Liaoning Chem Ind, 2006, 35(10):618-620.DOI:10.3969/j.issn.1004-0935.2006.10.021.
[19]	周荧, 武玉祥, 李永霞, 等. 高效液相色谱测定蓝莓叶片内源激素的方法研究[J]. 中国果树, 2018(3):88-91,94.
	ZHOU Y, WU Y X, LI Y X, et al. High performance liquid chromatography determination of endogenous hormones in blueberry leaves[J]. China Fruits, 2018(3):88-91, 94.DOI:10.16626/j.cnki.issn1000-8047.2018.03.023.
[20]	李华, 郭启高, 梁国鲁, 等. 利用高效液相色谱法同时测定枇杷果实中10种植物激素[J]. 西南大学学报(自然科学版), 2018, 40(3):18-25.
	LI H, GUO Q G, LIANG G L, et al. Simultaneous analysis of ten plant hormones in loquat fruitlet by high-performance liquid chromatogeaphy[J]. J Southwest Univ (Nat Sci Ed), 2018, 40(3):18-25.DOI:10.13718/j.cnki.xdzk.2018.03.003.
[21]	JIANG Y, ZHU Y, ZHANG L, et al. EjTFL1 genes promote growth but inhibit flower bud differentiation in loquat[J]. Front Plant Sci, 2020, 11:576. DOI: 10.3389/fpls.2020.00576
[22]	ZHANG X, ZHU J, CHENG S Y, et al. Effect of exogenous gibberellin on endogenous hormone and ginkgolide content in Ginkgo leaves[J]. Not Bot Horti Agrobo, 2020, 48(1):140-149. DOI:10.15835/nbha48111840.
[23]	徐雷, 贾飞飞, 王利琳. 拟南芥开花诱导途径分子机制研究进展[J]. 西北植物学报, 2011, 31(5):1057-1065.
	XU L, JIA F F, WANG L L. Progresses on molecular mechanisms of flowering transition in Arabidopsis[J]. Acta Bot Boreali Occidentalia Sin, 2011, 31(5):1057-1065.DOI:CNKI:SUN:DNYX.0.2011-05-031.
[24]	李巧峡, 张丽, 王玉, 等. 赤霉素调控植物开花及花器官发育的研究进展[J]. 中国细胞生物学学报, 2019, 41(4):746-758.
	LI Q X, ZHANG L, WANG Y, et al. The research progress of gibberellin on the regulation of flowering and floral organ development in plant[J]. Chin J Cell Biol, 2019, 41(4):746-758.DOI:10.11844/cjcb.2019.04.0026.
[25]	沙波. 内源激素、水分和氮磷钾含量与银杏大小年结实的关系[D]. 南宁: 广西大学, 2006.
	SHA B. The relationship between biennial fruiting and contents of endogenous hormones,moisture,nitrogen,phosphorus and potassium of Ginkgo biloba[D]. Nanning: Guangxi University, 2006.
[26]	张丽杰, 秦柏婷, 果冲, 等. 胡桃楸花芽形成过程中体内物质代谢变化的研究[J]. 沈阳农业大学学报, 2019, 50(3):280-288.
	ZHANG L J, QIN B T, GUO C, et al. Study on changes of material metabolism during flower bud formation of Juglans mandshurica[J]. J Shenyang Agric Univ, 2019, 50(3):280-288.DOI:10.3969/j.issn.1000-1700.2019.03.004.
[27]	徐红霞, 李晓颖, 陈俊伟. 枇杷花发育进程中氨基酸和碳水化合物代谢的变化[J]. 园艺学报, 2020, 47(2):233-241.
	XU H X, LI X Y, CHEN J W. Studies on the amino acid metabolism and carbohydrate metabolism variation during flower development in Eriobotrya japonicat[J]. Acta Hortic Sin, 2020, 47(2):233-241.DOI:10.16420/j.issn.0513-353x.2019-0271.
[28]	熊俊飞. 单性木兰雌雄株形态及理化性状差异研究[D]. 南宁: 广西大学, 2016.
	XIONG J F. Differences of morphological and physical and chemical properties of Kmeria septentrional is dandy male and female plants[D]. Nanning: Guangxi University, 2016.DOI: 10.7666/d.Y3087518.
[29]	胡玉玲, 姚小华, 张山, 等. 油茶花器官形成过程花芽与叶片生理生化变化[J]. 江西农业大学学报, 2016, 38(3):412-417.
	HU Y L, YAO X H, ZHANG S, et al. Physiological and biochemical change in flower buds and leaves during the formation of floral organs of Camellia oleifera[J]. Acta Agric Univ Jiangxiensis, 2016, 38(3):412-417.DOI:10.13836/j.jjau.2016059.
[30]	程华, 李琳玲, 王燕, 等. 银杏过氧化物酶基因POD1的克隆及表达分析[J]. 华北农学报, 2010, 25(6):44-51.
	CHENG H, LI L L, WANG Y, et al. Molecular cloning,characterization and expression of POD1 gene from Ginkgo biloba L.[J]. Acta Agric Boreali Sin, 2010, 25(6):44-51. DOI:10.7668/hbnxb.2010.06.009.
[31]	王双明, 李琼芳, 李庆. 雌雄银杏植株叶片生理生化特性的比较研究[J]. 农业现代化研究, 2003, 24(6):470-472.
	WANG S M, LI Q F, LI Q. Comparisons between female and male ginkgo’s physiological and biochemical characteristics[J]. Res Agric Mod, 2003, 24(6):470-472.DOI:10.3969/j.issn.1000-0275.2003.06.017.
[32]	温伟庆, 陈友吾. 银杏雌雄株过氧化物酶和过氧化氢酶活性差异研究[J]. 福建林业科技, 2002, 29(2):34-36,39.
	WEN W Q, CHEN Y W. The activity difference studies on the peroxidase and catalase of Ginkgo biloba female and male plants[J]. J Fujian For Sci Technol, 2002, 29(2):34-36,39.DOI:10.13428/j.cnki.fjlk.2002.02.009.
[33]	耿雅萍, 张鹏飞, 梁建萍, 等. 5种中药材中苯丙氨酸解氨酶(PAL)的生物信息学分析[J]. 河南大学学报(自然科学版), 2018, 48(6):669-682.
	GENG Y P, ZHANG P F, LIANG J P, et al. Bioinformatic analysis of PAL gene in five Chinese medicinal materials[J]. J Henan Univ (Nat Sci), 2018, 48(6):669-682.DOI:10.15991/j.cnki.411100.2018.06.005.
[34]	吴争荣. 甘肃产银杏总黄酮的考察与应用初步研究[D]. 兰州: 兰州大学, 2010.
	WU Z R. Studies on the total flavonoids and use of Ginkgo biloba L. in Gansu Province[D]. Lanzhou: Lanzhou University, 2010.DOI: 10.7666/d.Y1703574.
[35]	江德安. 叶用银杏品种黄酮含量的比较研究[J]. 湖北农业科学, 2004, 43(2):79-81.
	JIANG D A. A comparative study on the flavonoid contents of leaf ginkgo varieties[J]. Hubei Agric Sci, 2004, 43(2):79-81.

开花时期 flowering period	性别 gender	采样时间 sampling date	采样部位 sampling site
花芽分化后期 late flower bud differentiation	雄株	11月25日—次年3月2日(花粉母细胞分化期) 3月3日—3月16日左右(花粉粒形成期)	雌、雄株3~4年生短枝上(果枝)的饱满顶芽
花芽分化后期 late flower bud differentiation	雌株	11月25日—次年3月16日(珠被分化期或果芽生理分化后期)	雌、雄株3~4年生短枝上(果枝)的饱满顶芽
开花期 flowering period	雄株	3月17—4月7日	葇荑花序及周围小叶
开花期 flowering period	雌株	3月17—4月7日	胚珠及周围的小叶
花后期 late flowering	雄株	4月8日—4月28日	短枝顶芽原雄花序边的嫩叶
花后期 late flowering	雌株	4月8日—4月28日	胚珠周围的小叶

银杏雌、雄植株花芽分化后期及开花期间生理代谢的比较

Comparisons of physiological metabolism of female and male Ginkgo biloba trees during the late flower bud differentiation and flowering

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