关键词: 重组人碱性成纤维生长因子（rhbFGF）, 岛移植, 被膜, 尿病小鼠

Abstract:

Objective In this paper, we investigate the effect of islets transfected with adenovirus modified recombinant human basic fibroblast growth factor (rhbFGF) gene for angiogenesis after transplantation. Methods The adenoviral vector carrying rhbFGF-flag-GFP gene was transfected into the islets, and then transplanted into the renal capsule of a homologous diabetic mouse. The treatment of diabetes was observed within 30 days and the glucose tolerance test was performed at day 14 after transplantation. HE and immunohist-ochemistry staining were done to determine the islets function and the microvascular remodeling of the graft. Results The adenovirus modified rhbFGF enhanced efficiency of islet transfection, and there was no significant difference in activity between islets infected with a multiplicity of infection of 100 adenovirus and untreated after cultured for 60 h in vitro (P=0.75). The 200 IEQ islets transfected with rhbFGF-flag-GFP significantly increased islets function and accelerated the regeneration of blood vessels in vivo, which was better than the 400 IEQ islets transplantation alone. Conclusion Islets transfected with rhbFGF-flag-GFP gene will improve islets function and promote microvascular remodeling of the grafts, and which can reduce the amount of transplantation needed

Key words: recombinant human basic fibroblast growth factor (rhbFGF), slet transplantation, renal capsule, iabetic mouse