美国梾木(Cornus spp.)花粉活力及萌发特性

doi:10.11924/j.issn.1000-6850.casb16090024

中国农学通报 ›› 2017, Vol. 33 ›› Issue (28): 62-67.doi: 10.11924/j.issn.1000-6850.casb16090024

美国梾木(Cornus spp.)花粉活力及萌发特性

陈景震

湖南省林业科学院

收稿日期:2016-09-05 修回日期:2017-09-24 接受日期:2016-11-25 出版日期:2017-10-24 发布日期:2017-10-24
通讯作者: 陈景震
基金资助:
“十二五”国家科技支撑项目“新型油料能源植物选育及栽培示范”(2015BAD15B02)；湖南省林业科学院青年科研创新基金“美国梾木果实发育影响因子及机理研究”(2013LQJ12)。

Viability and Germination Characteristics of Cornus spp. Pollen Grain

Received:2016-09-05 Revised:2017-09-24 Accepted:2016-11-25 Online:2017-10-24 Published:2017-10-24

摘要/Abstract

摘要： 为了了解美国梾木花粉活力及萌发特性,采用扫描电镜技术和方差分析对13个美国梾木无性系花粉形态进行观测,并采用荧光显微技术和TTC染色法对无性系2号花粉萌发特性及花粉活力进行观察。结果显示：(1)不同无性系间花粉大小差异不显著,但外壁纹饰差异较大,极面沟距的变异系数最高且遗传最为稳定。(2)花序开花率为75%时花粉活力最高,整花4°C条件下短期保存花粉活力较高。(3)授粉后,多数花粉可在柱头上正常萌发,72 h后花粉管即可进入子房。研究认为,花粉外壁及极面沟距可作为鉴定美国梾木无性系的形态指标,美国梾木花粉活力及萌发特性是抗逆及丰产杂交组合的重要保证。

关键词: 流入人口, 流入人口, 迁入意愿, 影响因素, 中小城市

Abstract: In order to breed high-yield species,pollen grains of 13 Cornus spp. Wanyer clones were observed using scanning electron microscope and analyzed using analysis of variance. Pollen germination and pollen viability of clone 2 were viewed using fluorescence microscope and TTC staining method. The results showed that: (1)The size of pollen grains among different clones were similar,but the exine sculpture were variable. The variation coefficient of the furrow spacing in polar view was high and the inheritance was the most stable. (2)The pollen viability was highest when 75% of the inflorescence flowered. Whole flowers stored at 4°C for one month had higher pollen viability. (3)Most of the pollen grains could germinate on stigma,and their pollen tubes could enter the ovary in 72 h after pollination. In conclusion,exine sculpture and the furrow spacing could be used as morphological indexes to identify different clones. Pollen germination and pollen viability were important to breeding program.

陈景震. 美国梾木(Cornus spp.)花粉活力及萌发特性[J]. 中国农学通报, 2017, 33(28): 62-67.

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美国梾木(Cornus spp.)花粉活力及萌发特性

Viability and Germination Characteristics of Cornus spp. Pollen Grain

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