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中国农学通报 ›› 2016, Vol. 32 ›› Issue (18): 37-42.doi: 10.11924/j.issn.1000-6850.casb16010034

所属专题: 玉米

• 农学 农业基础科学 • 上一篇    下一篇

玉米南方锈病菌的超微结构研究

邢国珍1,李晶晶1,肖继斌1,焦志鑫1,刘 娜1,韩青梅2,郑文明1   

  1. (1河南农业大学生命科学学院/小麦玉米作物学国家重点实验室/河南粮食作物协同创新中心,郑州 450002;2西北农林科技大学植物保护学院/旱区作物逆境生物学国家重点实验室,陕西杨凌 712100)
  • 收稿日期:2016-01-08 修回日期:2016-06-08 接受日期:2016-03-15 出版日期:2016-06-28 发布日期:2016-06-28
  • 通讯作者: 郑文明
  • 基金资助:
    河南省科技创新杰出人才计划“利用基因沉默技术创制抗锈新种质”(201513);河南省重大公益性科研项目“主要农作物重大病虫害成灾机理及可持续控制技术研究应用”(08004)。

Ultrastructure Investigation of Southern Corn Rust Fungus (Puccinia polysora Underw)

Xing Guozhen1, Li Jingjing1, Xiao Jibin1, Jiao Zhixin1, Liu Na1, Han Qingmei2, Zheng Wenming1   

  1. (1College of Life Science, Henan Agricultural University/State Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops, Zhengzhou 450002;2College of Plant Protection, Northwest Agriculture and Forestry University/State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling Shannxi 712100)
  • Received:2016-01-08 Revised:2016-06-08 Accepted:2016-03-15 Online:2016-06-28 Published:2016-06-28

摘要: 认识玉米南方锈菌的超微结构特征和侵染过程的细胞学特征,可为南方锈菌致病机理和玉米抗锈机制研究提供重要依据。本研究采集玉米品种‘郑丹958’和‘先玉335’自然发病的南方锈病病叶,4%戊二醛固定液抽气固定,采用扫描电镜和透射电镜技术进行观察。结果表明,玉米南方锈病菌的胞间菌丝呈丝状,胞间菌丝和胞内菌丝细胞核在不同感病玉米品种之间具有单核或者双核的差异;胞间菌丝与寄主细胞接触诱导吸器母细胞的分化,吸器母细胞穿透寄主细胞壁在细胞内形成吸器;寄主细胞质膜在吸器的侵入部位内陷,随着吸器的发育,在吸器颈部周围形成胼胝质并将吸器颈部完全包围起来;夏孢子表面具密致、交错排列的刺突,刺突基部外围存在圆形凹陷的脊。本研究揭示了玉米南方锈菌基本超微结构特征和侵染机构(吸器)细胞学特征,并对其在不同品种细胞内的核相进行了分析,为玉米南方锈菌组织病理学研究奠定重要基础。

关键词: 留茬高度, 留茬高度, 土壤呼吸速率, 土壤有机碳, 燕麦

Abstract: The investigation on ultrastructural features and cytological features of infection process for southern corn rust fungus (Puccinia polysora Underw) would provide an important basis for understanding its pathogenesis and rust resistance mechanisms of maize. The natural infected corn leaves of southern rust fungus from two maize varieties ‘Zhengdan 958’ and ‘Xianyu 335’ were collected and the samples were vacuumed and immobilized using 4% glutaraldehyde. The processed samples were then investigated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results indicated that: the intercellular hyphae of southern corn rust fungus were filiform. The intercellular hyphae and intracellular hyphae had difference in monokaryon or dicaryon. The contact between the intercellular hyphae and the host cell induced the differentiation of haustorial mother cell. The haustoria appeared in the host cell after haustorial mother cell penetrating through the host cell wall. The host cytoplasmic membrane was invaginated in the place where the haustoria invaded into the host cell. The callus was generated to surround the haustoria neck with the development of haustoria. The urediospore had the numerous staggered spines on the surface. The rounded and recessed crista existed on the outside of the spines. The study offered the ultrastructural features and cytological features of infected institutions (haustoria) for southern corn rust fungus, and analyzed the nuclear phases among the different varieties and laid a foundation for the histopathology of southern corn rust fungus.

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