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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (27): 171-176.doi: 10.11924/j.issn.1000-6850.casb16010021

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

DNS比色法测定土壤蔗糖酶的检测条件选择

李朝英,郑路   

  1. 中国林业科学研究院热带林业实验中心,中国林业科学研究院热带林业实验中心
  • 收稿日期:2016-01-06 修回日期:2016-08-25 接受日期:2016-04-25 出版日期:2016-09-29 发布日期:2016-09-29
  • 通讯作者: 郑路
  • 基金资助:
    广西自然科学基金“马尾松人工林采伐剩余物分解及提升地力研究”(2013GXNSFAA019106)。

Soil Sucrase: Detection Conditions Based on DNS Colorimetric

  • Received:2016-01-06 Revised:2016-08-25 Accepted:2016-04-25 Online:2016-09-29 Published:2016-09-29

PDF (PC)

633

摘要： 为了准确测定土壤蔗糖酶含量，比较分析了不同波长、显色剂加入量、甲苯加入量、加热时间、显色稳定时间等条件对3,5-二硝基水杨酸(3,5-dinitrosalicylic acid, DNS)比色法测定土壤蔗糖酶含量的影响。结果表明，当波长为510 nm，显色剂用量为1.5 ml，甲苯加入量0.1 ml，显色反应的水浴时间5 min，待测液存放时间小于4 h，显色稳定时间在0.5-2 h之间时，标准曲线在0.25-2mg范围内线性良好(R2=0.9991)，土壤蔗糖酶含量的变异系数(CV)小于9%，回收率在96%~109%范围内，检测结果的精密度和准确性较高。上述检测方法安全、经济、高效，适用于实验室批量土壤的蔗糖酶含量测定。

关键词: 养殖水塘, 养殖水塘, 溶解氧, 水呼吸, 鱼呼吸, 泛塘

Abstract: The aim was to detect the content of soil sucrase precisely. The effect of different wavelengths, the amount of chromogenic agent and toluene, heating time and the stable time of color on the content of soil sucrase detected by 3,5-2 dinitrosalicylic acid (DNS) method was analyzed. The results showed that when the wavelength was 510 nm, the amount of reagent was 1.5 mL, the amount of toluene was 0.1 mL, the time of water bath was 5 min, storage time of the solution to be determined was less than 4 h, the stable time of color was between 0.5-2 h, the standard curve in the range of 0.25-2 mg had good linear relation (R2=0.9991), the coefficient of variation (CV) was less than 9% , the recoveries were in the range of 96%- 109% , and the precision and accuracy of the test results were relatively high. The above method was safe, economical and efficient, and suitable for batch determination of soil sucrase.