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中国农学通报 ›› 2016, Vol. 32 ›› Issue (15): 93-97.doi: 10.11924/j.issn.1000-6850.casb15110011

所属专题: 生物技术 油料作物 农业气象

• 生物技术科学 • 上一篇    下一篇

干旱胁迫对春大豆超氧化物歧化酶活性和丙二醛含量的影响

李雪凝,董守坤,刘丽君,徐亚会,高鑫宇,王利彬   

  1. 东北农业大学 农学院,东北农业大学 农学院,东北农业大学 农学院,东北农业大学 农学院,东北农业大学 农学院,东北农业大学 农学院
  • 收稿日期:2015-11-02 修回日期:2016-05-03 接受日期:2015-12-24 出版日期:2016-06-01 发布日期:2016-06-01
  • 通讯作者: 董守坤
  • 基金资助:
    国家科技支撑计划“大豆高产高效关键技术研究与示范”(2014BAD11B01);教育部博士点基金“阶段性干旱胁迫下春大豆生理响应机制及对产质量影响的研究”(20122325120013)。

Effect of Drought Stress on SOD Activity and MDA Content of Spring Soybean

  • Received:2015-11-02 Revised:2016-05-03 Accepted:2015-12-24 Online:2016-06-01 Published:2016-06-01

摘要: 大豆是一种对水分最敏感的豆类作物,明确干旱对大豆叶片主要生理特性的影响,对于进行更好的水分管理具有重要的理论和实践意义。本试验选用耐旱型品种黑农44和敏感型品种黑农65为材料,测定了不同生育时期干旱及复水后大豆叶片SOD活性和MDA含量。结果表明:在干旱条件下,SOD活性随干旱程度增加呈先增加后降低的趋势,活性增加幅度花期>苗期>鼓粒期,且黑农44的SOD活性明显大于黑农65;MDA含量随干旱程度增加呈逐渐上升趋势,苗期>开花期>鼓粒期,且黑农44明显小于黑农65。复水后,SOD活性和MDA含量均逐渐降低;苗期干旱复水后MDA含量恢复较快,开花期干旱复水后恢复时间较长,鼓粒期干旱复水后不能恢复。

关键词: 作物生长季干湿状况, 作物生长季干湿状况, 空间分布, 时间演变, 黄土高原

Abstract: Soybean is one of the most sensitive to water in legume crops. Clearing the effect of drought stress on leaf physiological characteristics of soybean is an important theoretical and practical significance for better managing water .This test measured the leaf SOD activity and MDA content at different growth stages under the condition of drought stress and consequent re-watering, choosing the drought-resistant variety(Heinong 44) and the sensitive variety(Heinong 65) as materials. The results suggested that SOD activity initially increased and then decreased with increasing water stress , further the rate of increase was that the flowering stage >the seedling stage >the filling stage .SOD activity of Heionng 44 is significantly stronger than Heinong 65 . Increasing drought stress MDA content initially increased. The rate of increase was that the seedling stage >the flowering stage >the filling stage and the MDA content of Heinong 44 obviously lower than Heinong 65. After re-watering,SOD activity and MDA content of both cultivars gradually decreased approaching the control treatment. Whereas re-watering at seeding stage was better for MDA content to recover than at flowering stage.MDA content can’t recover when re-watering at filling stage.