低温胁迫对转高山离子芥CbPLDα、CbPLDβ基因烟草抗氧化酶系统的影响

doi:10.11924/j.issn.1000-6850.casb15060098

中国农学通报 ›› 2015, Vol. 31 ›› Issue (34): 209-215.doi: 10.11924/j.issn.1000-6850.casb15060098

所属专题：生物技术；烟草种植与生产

低温胁迫对转高山离子芥CbPLDα、CbPLDβ基因烟草抗氧化酶系统的影响

陈霞,李宜珅,仇奕之,何文平,杨宁

(西北师范大学生命科学学院,兰州 730070）

收稿日期:2015-06-17 修回日期:2015-11-17 接受日期:2015-10-08 出版日期:2015-12-17 发布日期:2015-12-17
通讯作者: 杨宁
基金资助:
国家自然科学基金“高山离子芥细胞磷脂酶D在植物抗寒生理生态中的调控机制研究”(31160087)；国家自然科学基金“磷脂酶响应二萜类化感物质作用的分子及生理调控机制研究”(31360061)。

Effect of Low Temperature Stress on Antioxidant System of CbPLDα and CbPLDβ Transgenic Tobaccos

Chen Xia, Li Yishen, Qiu Yizhi, He Wenping, Yang Ning

(College of Life Science, Northwest Normal University, Lanzhou 730070)

Received:2015-06-17 Revised:2015-11-17 Accepted:2015-10-08 Online:2015-12-17 Published:2015-12-17

摘要/Abstract

摘要： 为研究高山离子芥CbPLDα、CbPLDβ 转基因烟草对低温胁迫的生理响应，采用4、0℃低温胁迫，揭示了CbPLDα、CbPLDβ 基因在植物抵抗低温中的作用。结果表明：随着4℃胁迫时间的延长，野生型烟草和转基因烟草的丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性以及抗坏血酸过氧化物酶(APX)活性均呈增加趋势，过氧化物酶(POD)和过氧化氢酶(CAT)活性则呈先上升后下降趋势。0℃胁迫条件下，野生型烟草和转基因烟草的丙二醛(MDA)含量和过氧化氢酶(CAT)活性以及抗坏血酸过氧化物酶(APX)均先上升后下降，过氧化物酶(POD)和超氧化物歧化酶(SOD)活性呈增加趋势。CbPLDα、CbPLDβ 转基因活性烟草的MDA含量显著低于野生型烟草植株，而抗氧化酶活性显著高于野生型烟草对照，且转基因烟草对0℃低温胁迫的响应更积极敏感。CbPLDα、CbPLDβ 基因的导入提高了烟草幼苗的抗低温能力。

关键词: 壶天山羊, 壶天山羊, 湘东黑山羊, 肉质特性, 肉成份

Abstract: To study the physiological response of Chorispora bungeana CbPLDα and CbPLDβ transgenic tobacco to low temperature, the authors used 4℃ and 0℃ temperature stresses to reveal the role of CbPLDα and CbPLDβ in low temperature resistance in plants. The results showed that the content of MDA, the activity of SOD and APX increased under 4℃ treatment, while the activity of POD and CAT decreased with the time. However, under 0℃ treatment, the content of MDA, the activity of CAT and APX decreased with the time, while the activity of SOD and POD increased. Under the cold stress, the activities of POD, SOD and CAT in transgenic tobacco plants were obviously higher than those in the non-transgenic tobacco plants. The contents of MDA in transgenic tobacco plants were obviously lower than those in the non-transgenic tobacco plants. The results showed that the over expressing of CbPLDα and CbPLDβ gene improved the cold resistance of tobacco.

中图分类号:

陈霞,李宜珅,仇奕之,何文平,杨宁. 低温胁迫对转高山离子芥CbPLDα、CbPLDβ基因烟草抗氧化酶系统的影响[J]. 中国农学通报, 2015, 31(34): 209-215.

Chen Xia,Li Yishen,Qiu Yizhi,He Wenping and Yang Ning. Effect of Low Temperature Stress on Antioxidant System of CbPLDα and CbPLDβ Transgenic Tobaccos[J]. Chinese Agricultural Science Bulletin, 2015, 31(34): 209-215.

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低温胁迫对转高山离子芥CbPLDα、CbPLDβ基因烟草抗氧化酶系统的影响

Effect of Low Temperature Stress on Antioxidant System of CbPLDα and CbPLDβ Transgenic Tobaccos

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