井冈霉素的研究进展

doi:10.11924/j.issn.1000-6850.casb15020060

摘要/Abstract

摘要： 井冈霉素是最成功的生物源杀菌剂之一，也是防治水稻纹枯病的最常用与最有效的农药之一。文章综述了井冈霉素的理化性质及关于井冈霉素生物活性的研究，井冈霉素不仅抑制海藻糖酶活性，而且还具有抑制真菌的纤维素降解酶、多聚半乳糖醛酸酶和肌醇生物合成的活性；总结了井冈霉素的检测方法，通过色谱与质谱联用等方法来完成，最低检测量达到了0.4 ng。提出井冈霉素的生物合成是先将葡萄糖经过戊糖磷酸途径生成景天庚酮糖-7-磷酸，然后将葡萄糖与景天庚酮糖-7-磷酸经过10个酶催化，最终合成了井冈霉素A。最后评价了井冈霉素的发酵生产工艺，经过培养基组分优化、补料添加、发酵条件控制与分离工艺改进，井冈霉素的生产工艺也日趋完善。

关键词: 闽楠, 闽楠, 播种育苗, 遮荫网

Abstract: Jinggangmycin is one of the most successful bio-pesticides and is mainly used to protect Oryza sativa against Rhizoctonia solani. This paper reviewed the physicochemical property of jinggangmycin and the researches about the bioactivity of Jinggangmycin. jinggangmycin not only inhibited the activity of trehalase, cellulose-degrading enzymes and polygalacturonase but also affected the biosynthesis of inositol. Then the author summed up the detection method of Jinggangmycin. The lowest limit of jinggangmycin that could be detected was 0.4 ng with the LC-MS or GC-MS method. Then biosynthesis pathway of jinggangmycin was put forward, which was producing sedoheptulose-7-phosphate using glucose by pentose phosphate pathway and then catalyzing glucose and sedoheptulose-7-phosphate by 10 enzymes. The manufacturing technique of jinggangmycin has been greatly improved with the optimization of media components and feed-batch, the control of fermentation conditions and the separation process.

徐利剑,赵维,曹聪,王健,王茜,王庆贵. 井冈霉素的研究进展[J]. 中国农学通报, 2015, 31(22): 191-198.

Xu Lijian,Zhao Wei,Cao Cong,Wang Jian,Wang Qian and Wang Qinggui. Research Progress of Jinggangmycin[J]. Chinese Agricultural Science Bulletin, 2015, 31(22): 191-198.

参考文献

[1] Iwasa T, Yamamoto H, Shibata M. Studies on validamycins, new antibiotics. I. Streptomyces hygroscopicus var. limoneus nov. var., validamycin-producing organism[J]. The Journal of antibiotics,1970,23(12):595-602.
[2] 上海市农药研究所.井冈霉素筛选的研究[J].上海化工,1974(4):14-19.
[3] 周祥.井冈霉素生物合成限速步骤解析及高产[D].上海:上海交通大学,2011:1-2.
[4] Wei Z, Bai L, Deng Z X, et al. Enhanced production of validamycin A by H₂O₂-induced reactive oxygen species in fermentation of Streptomyces hygroscopicus 5008[J]. Bioresource Technology,2011,102,1783-1787.
[5] Almabruk K H, Asamizu S, Chang A, et al. The alpha-Ketoglutarate/Fe-II-Dependent Dioxygenase VldW is Responsible for the Formation of Validamycin B[J]. Chembiochem,2012,13(15):2209-2211.
[6] Gams W, van Laar W. The Use of Solacol (Validamycin) as a Growth Retardant in the Isolation of Soil Fungi[J]. Netherlands Journal of Plant Pathology,1982,88(2):39-45.
[7] 张国忠.井冈霉素防治水稻纹枯病田间药效试验[J].现代农业科技,2009(6):104.
[8] 汤少云,陈天松,朱盛兰,等.井冈霉素防治水稻纹枯病、稻曲病试验[J].湖北植保,2010(4):47-48.
[9] 吴汉章.井冈霉素防治小麦纹枯病试验初报[J].江苏农业科学,1981(1):36-38.
[10] 李顺德,黄业修,吴桂珍.井冈霉素防治玉米穗腐病试验[J].Pesticides,1997,36(1):38-39.
[11] 李顺德,黄兰英.井冈霉素防治玉米纹枯病的一种施药新技术[J].农药,1999,38(1):35.
[12] 伍佐君.井冈霉素防治柑桔脚腐病效果好[J].农业科技通讯,1991(5):37.
[13] 申屠旭萍,郑裕国,俞晓平.井冈霉素及其分解产物的开发利用[J].国外医药:抗生素分册,2005(6):275-278.
[14] Muller J, Boller T, Wiemken A. Effect s of validamycin A, a potent trehalase inhibitor and phytohormones on trehalose metabolism in roots and root nodules of soybean and cowpea[J]. Planta,1995,97(2):362-368.
[15] Wakai O, Matsuura K. Characteristics of Validamycin as a fungicide for Rhizoctonia disease control[J]. Review of Plant-Protection-Research,1975(8):81-92.
[16] 李明海,杨迎青,杨媚,等.井冈霉素对水稻纹枯病菌细胞壁降解酶活性和可溶性蛋白的影响[J].华中农业大学学报,2010,29(3):272-276.
[17] Copping L G, Menn J J. Biopesticides: a review of their action, applications and efficacy[J]. Pest Manag Sci,2000,56(8):651-676.
[18] 张穗,郭永霞,唐文华,等.井冈霉素A对水稻纹枯病菌的毒力和作用机理研究[J].农药学学报,2001,3(4):31-37.
[19] 张穗,赵清华,唐文华,等.井冈霉素A对水稻抗性相关酶活性的影响[J].植物保护学报,2003,30(2):177-180.
[20] 周明国,叶钟音,刘经芬.稻纹枯病菌对井冈霉素抗药性检测、监测和诱导[J].中国水稻科学,1991,5(2):73-78.
[21] 金梅松,蒋文烈.浙江省水稻纹枯病菌对井冈霉素的抗药性测定[J].浙江农业学报,1997,9(3):127-130.
[22] 孙海燕,丁晓菲,杜文珍,等.江苏、河南、安徽和山东四省小麦纹枯病菌对井冈霉素的敏感性监测[J].农药学学报,2011,13(6):653-656.
[23] 潘云枫,张赛玲,许彩芬.水稻纹枯病对井冈霉素的抗性监测研究[J].上海农业科技,2012(2):128.
[24] 黄昌华,杨丹,姚学英.井冈霉素——不易产生抗性的抗菌素[J].湖北植保,1994(2):18-19.
[25] 夏晓明,王开运,王怀训,等.禾谷丝核菌对井冈霉素的抗性风险预测[J].农药学学报,2006,8(2):115-120.
[26] 胡燕,王怀训,夏晓明,等.四地区小麦纹枯病对6种杀菌剂的抗性比较[J].植物保护学报,2006,33(4):423-427.
[27] 许文耀,吕伟成,胡秀荣.水稻纹枯病菌抗井冈霉素突变体的生物学特性[J].福建农林大学学报:自然科学版,2007,36(3):230-233.
[28] 杜良伟,徐秋红,王彦辉.井冈霉素在水稻和土壤上残留及消解动态[J].农药,2012,51(4):284-286.
[29] Iwasa T, Yamamoto H, Shibata M. Studies on validamycins, new antibiotics. II. Production and biological properties of validamycin A and B[J]. The Journal of Antibiotics,1971,24:107-113
[30] 印桂华,黎树荣.井岗霉素农药的毒性研究[J].卫生毒理学杂志,1992(2):101.
[31] 王家德,钱海丰,胡宝兰,等.井冈霉素对土壤可培养微生物的生态效应[J].农药,2006,45(11):771-774.
[32] 王志烨,徐茜,钱海丰.FDA水解检测井冈霉素的土壤微生物活性效应[J].土壤通报,2007,38(5):1030-1032.
[33] 沈寅初.有效霉素的化学[J].农药译丛,1984,6(1):11-17.
[34] He J, Chen S, Ruan L, et al. Determination of the Fungicide Validamycin A by Capillary Zone Electrophoresis with Indirect UV Detection[J]. Agric Food Chem,2003,51(26):7523-7527.
[35] 上海市农药研究所.井冈霉素效价测定的两种方法[J].微生物学通报,1977(4):13-14.
[36] 张存政,何丹军,骆爱兰,等.井岗霉素在水稻叶片中的残留分析[J].南京农业大学学报,2005,28(2):107-111.
[37] 余晓江,吴萍,韩志华,等.井冈霉素在大米及稻壳中的残留研究[J].现代农药,2011,10(2):37-39.
[38] 涨汀州.食品中井冈霉素残留分析方法的建立[D].福建:福建农林大学,2000:10-13.
[39] 关文碧,王晨,张红艳.高效液相色谱-串联质谱法测定稻米和稻壳中井冈霉素A的残留[J].分析科学学报,2012,28(1):136-138.
[40] 许鹏军,战瑞,陶哺,等.柱前衍生化毛细管气相色谱法测定土壤中井冈霉素A残留[J].分析化学,2008,36(2):29-252.
[41] Dong H, Mahmud T, Tornus I, et al. Biosynthesis of the validamycins: identification of intermediates in the biosynthesis of validamycin A by Streptomyces hygroscopicus var. limoneus[J]. J Am Chem Soc,2001,123:2733-2742.
[42] Bai L, Li L, Xu H, et al. Functional analysis of the validamycin biosynthetic gene cluster and engineered production of validoxylamine A[J]. Chem Biol,2006,13(4):387-397.
[43] Singh D, Kwon H J, Rajkarnikar A, et al. Glucoamylase gene, vldI, is linked to validamycin biosynthesis in Streptomyces hygroscopicus var. limoneus, and vldADEFG confers validamycin production in Streptomyces lividans, revealing the role of VldE in glucose attachment[J]. Gene,2007,395(1):151-159.
[44] Singh D, Seo M J, Kwon H J, et al. Genetic localization and heterologous expression of validamycin biosynthetic gene cluster isolated from Streptomyces hygroscopicus var. limoneus KCCM 11405 (IFO 12704)[J]. Gene,2006,376(1):13-23.
[45] Minagawa K, Zhang Y, Ito T, et al. ValC, a new type of C₇-Cyclitol kinase involved in the biosynthesis of the antifungal agent validamycin A[J]. Chembiochem,2007,8(6):632-641.
[46] Xu H, Zhang Y, Yang J, et al. Alternative epimerization in C₇N-aminocyclitol biosynthesis is catalyzed by ValD, a large protein of the vicinal oxygen chelate superfamily[J]. Chem Biol,2009,16(5):567-576.
[47] Yang J, Xu H, Zhang Y, et al. Nucleotidylation of unsaturated carbasugar in validamycin biosynthesis[J]. Org Biomol Chem,2010,9(2):438-449.
[48] Asamizu S, Yang J, Almabruk K H, et al. Pseudoglycosyl transferase catalyzes nonglycosidic C-N coupling in validamycin a biosynthesis[J]. J Am Chem Soc,2011,133(31):12124-12135.
[49] 陈少莲,倪观荣,候玉芳.井冈霉素高产菌株亚硝酸诱变筛选的研究[J].浙江农业科学,1989(6):271-274.
[50] 陈力力,鲁迎新.微波诱变选育井冈霉素高产菌[J].生物技术,2003,13(5):14-15.
[51] 邹坚.应用原生质体诱变技术筛选井冈霉素[J].现代农药,2003,2(2):27-28.
[52] 虞龙,安肖.N⁺和Ti⁺离子注入井冈霉素生产菌诱变筛选高产菌株的研究[J].辐射研究与辐射工艺学报,2007,25(6):371-374.
[53] 牛莉娜,葛翠凤,葛绍荣.链霉素抗性筛选井冈霉素高产菌株的研究[J].安徽农业科学,2007,35(28):8930-8932.
[54] 安肖,虞龙.钛离子注入井冈霉素生产菌株的诱变效应[J].生物加工过程,2008,6(2):56-60.
[55] 廖晓珣,陶科,侯太平.井冈霉素产生菌的诱变选育及发酵条件优化[J].四川大学学报:自然科学版,2008,45(4):963-966.
[56] 张茜茜,朱永强,刘华梅,等.井冈霉素高产菌株的复合诱变筛选[J].湖北农业科学,2009,48(3):612-614.
[57] 赵南,吴冬兰,唐洪啟,等.氮离子注入井冈霉素产生菌诱变高产菌株的研究[J].江西农业学报,2010,22(4):122-123.
[58] 覃敬羽,李世杰,刘华梅.井冈霉素高产菌株原生质体诱变筛选研究[J].农业基础科学,2010,22:38-39.
[59] 丁艳,郑之明,王丽,等.N⁺注入诱变选育井冈霉素A高产菌的研究[J].工业微生物,2013,43(6):18-22.
[60] 钟世华,徐满才,徐广宇.正交法优化井冈霉素培养基研究[J].湖南师范大学自然科学学报,2008,31(1):69-71.
[61] 上海农药所.井冈霉素[M].上海:上海市人民出版社,1977:60-63.
[62] 陈久仁.影响井冈霉素发酵效价、生产力和碳素转化因素的探讨[J].浙江农业大学学报,1992(4):137-141.
[63] 汪世山,陈守文,喻子牛.吸水链霉菌井冈霉素发酵中氮源及碳氮比的优选[J].华中农业大学学报,2001,20(6):554-556.
[64] 廖悦乔.发酵温度对有效霉素生物合成的影响[D].上海:上海交通大学,2008:12-13.
[65] 沈赤.井冈霉素水溶性粉剂制备研究[J].农药,1984(4):8-9.
[66] 刘义雄,张文宣,周圣骄,等.流加补氨水代替碳酸钙在井冈霉素生物发酵中的研究[J].化学与生物工程,2013,30(11):23-25.
[67] 陈明兆.井冈霉素发酵过程补糖工艺优化[J].发酵科技通讯,2014,43(1):4-5.
[68] 郑裕国,虞炳钧,陈小龙,等.离子交换法提取井冈霉素的研究[J].农药,1996,35(11):9-11.
[69] 杨志钧,殷瑜,洪文荣,等.大孔阳离子交换树脂对井冈霉素的吸附研究[J].离子交换与吸附,2005,21(6):551-556.
[70] 钟世华,齐风佩,范明亮,等.D151树脂一步法提取井冈霉素[J].湖南师范大学自然科学学报,2010,33(1):71-74.
[71] 曹晖,王绍云.井冈霉素的提纯和含量测定研究[J].安徽农业科学,2011,39(6):3359-3361.
[72] 白林泉,邓子新.微生物次级代谢产物生物合成基因簇与药物创新[J].中国抗生素杂志,2006,31(2):80-87.