邻苯二酚降解菌筛选及其降解特性

doi:10.11924/j.issn.1000-6850.2013-3061

中国农学通报 ›› 2014, Vol. 30 ›› Issue (26): 165-169.doi: 10.11924/j.issn.1000-6850.2013-3061

• 资源环境生态土壤气象 • 上一篇下一篇

邻苯二酚降解菌筛选及其降解特性

程珂珂刘雯卫亚红

收稿日期:2013-11-21 修回日期:2014-01-03 出版日期:2014-09-15 发布日期:2014-09-15
基金资助:
陕西省国际科技合作重点项目“基于全基因组测序的布洛芬降解调节因子基因定位研究”(2013KW-29)；西北农林科技大学科技创新专项“布洛芬高效降解菌株降解基因功能分析”(QN2011113)；国际科技合作项目“干旱半干旱地区药物及个人护理品类污染物微生物降解技术”(A213021202)。

Screening and Degradation Characteristics of Catechol Degradable Bacteria

Received:2013-11-21 Revised:2014-01-03 Online:2014-09-15 Published:2014-09-15

摘要/Abstract

摘要： 为了缓解环境中邻苯二酚的污染，筛选并研究邻苯二酚降解菌，以期为邻苯二酚污染的微生物修复提供备选菌株。从西安污水处理厂水样及陕西苹果园土壤中分离以邻苯二酚为唯一碳源的降解细菌。采用以邻苯二酚为唯一碳源的无机盐培养液，在28℃及150 r/min的恒温摇床中对菌株进行培养，通过测定培养液中剩余的邻苯二酚浓度，计算菌株对邻苯二酚的表观降解率，并通过pH、装样量和浓度3方面对菌株降解特性进行研究。结果表明，在pH 6，装样量50 mL，浓度100 mg/L时，菌体的生长量以及邻苯二酚的降解率达到最大，该菌株显示出良好的应用前景。

关键词: 作用机理, 作用机理

Abstract: In order to alleviate the environmental pollution of catechol, the screening and research of catechol degradable bacteria were supposed to provide alternative strains for the bioremediation polluted by catechol. The author focused on the enrichment, isolation and degradation characteristics of catechol degradable bacteria. Catechol was used as a sole source of carbon in the cultural medium, the degradable bacteria was isolated and screened from Xi’an sewage treatment plant water and Shaanxi apple orchard soil. After shaking at 28℃and 150 r/min in the inorganic salt liquid medium, in which catechol was used as a sole source of carbon, the apparent degrading rates of the bacteria for degradation of catechol was tested by the concentration of catechol in residue medium. The pH, volume and concentration were optimized to observe the influence of catechol degradation characteristics. Based on the test, it could draw a conclusion that the growth of bacteria for degradation of catechol and the degrading rates were reaching extreme when pH was 6, volume was 50 mL and concentration was 100 mg/L. This strain showed a good application prospect.

程珂珂刘雯卫亚红. 邻苯二酚降解菌筛选及其降解特性[J]. 中国农学通报, 2014, 30(26): 165-169.

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邻苯二酚降解菌筛选及其降解特性

Screening and Degradation Characteristics of Catechol Degradable Bacteria

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