欢迎访问《中国农学通报》,

中国农学通报 ›› 2013, Vol. 29 ›› Issue (16): 79-83.doi: 10.11924/j.issn.1000-6850.2012-4082

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

转ER-sHSP基因番茄植株的耐盐性检测

赵春梅 刘箭   

  • 收稿日期:2012-12-19 修回日期:2013-02-28 出版日期:2013-06-05 发布日期:2013-06-05
  • 基金资助:
    青岛农业大学高层次人才启动基金

Determination of Salt-tolerance of Transgenic Tomato with ER-sHSP Gene

  • Received:2012-12-19 Revised:2013-02-28 Online:2013-06-05 Published:2013-06-05

摘要: 热激蛋白在植物应对各种环境胁迫的应答中执行重要的功能并存在交叉保护现象。前期结果表明内质网小分子热激蛋白(ER-sHSP)的过量表达能够显著增强转基因番茄的抗冷能力,为继续深入研究ER-sHSP的生理功能,本文对高盐胁迫下转ER-sHSP基因番茄的耐受性进行了检测,结果显示:与对照相比,转基因番茄抗性表型明显,持有较高的植物干鲜重,脯氨酸含量大幅度提高,SOD酶活性增加明显,而MDA含量和电导率则较低。说明ER-sHSP的过量表达提高了转基因番茄的抗盐胁迫能力

关键词: 远红外成像, 远红外成像

Abstract: It is well known that plant heat shock proteins (HSPs) play important roles in response to adverse environmental conditions and there are cross-resistant mechanisms in their functions. We previously demonstrated that overexpressing ER-sHSP in transgenic tomato highly enhanced their abilities against cold stress. To continue improve our understanding about the physiological function of ER-sHSP, we assessed the salt-resistant ability of transgenic tomato plants under salt stress. Results showed that transgenic lines possessed higher fresh and dry weight, more proline content, higher SOD activity, lower MDA content, and lower relative electrical leakage. Transformed lines showed obvious resistance to salt stress compared to the non-transgenic lines. These results indicated that overexpression of ER-sHSP enhanced tolerance of transgenic tomato plants to salt stress.