外源NO对UV-B胁迫下小麦幼苗生长、活性氧组分和光合特性的影响

doi:10.11924/j.issn.1000-6850.2012-2295

摘要/Abstract

摘要： 为了研究外源NO对UV-B胁迫下小麦幼苗生长、活性氧组分以及光合特性的影响，以ML7113小麦为受试材料，以硝普钠(Sodium nitroprusside，SNP)作为外源NO的供体，预先用0.1 mmol/L的SNP浸种24 h，设置4个处理组：对照组(CK)、紫外线UV-B胁迫处理组(B)、UV-B胁迫和SNP复合处理组(B+SNP)、SNP单独处理组。待幼苗生长7天后取其叶片进行可溶性糖、可溶性蛋白、脯氨酸(Pro)、丙二醛(MDA)、超氧阴离子(O₂^-)、过氧化氢(H₂O₂)、气孔密度、光合色素及叶绿素荧光参数等指标的测定。结果表明，NO供体SNP能显著增加经UV-B胁迫后小麦幼苗的株高，并使其小麦幼苗可溶性蛋白、可溶性糖、脯氨酸含量分别提高19.69%、15.25%、41.36%，同时使其小麦叶正面气孔密度增大10.34%。SNP单独处理使得光合色素（叶绿素和类胡萝卜素）的含量较CK分别提高12.43%和5.99%，叶绿素荧光参数Fv/Fm和Fv/F0较CK显著提高0.5%和5.16%。此外，SNP能使小麦幼苗丙二醛(MDA)、过氧化氢(H₂O₂)的含量较CK显著降低27.30%和74.92%，超氧阴离子(O₂^-)的产生速率较CK显著降低24.47%。通过体内相关生理指标物质含量的变化来响应UV-B胁迫对小麦幼苗造成的伤害，而适宜浓度的外源NO(0.1 mmol/L)可以缓解UV-B胁迫下对小麦幼苗的伤害作用，从而增强小麦幼苗对UV-B胁迫的适应能力。

关键词: 根系活力, 根系活力

Abstract: ML7113 wheat seedling was used for test materials for the purpose of investigating the effects of exogenous nitric oxide on wheat seedling growth, reactive oxygen species and photosynthetic characteristic under UV-B stress. Sodium nitroprusside (SNP) was selected as an exogenous nitric oxide (NO) donor, and the seed of wheat was pre-soaked with 0.1 mmol/L SNP for 24 h. Under the UV-B stress conditions, there were four treatment groups including control group (CK), ultraviolet ray UV-B stress treatment group (B), UV-B and sodium nitroprosside composite treatment group (B+SNP) and sodium nitroprosside individual treatment group (SNP). 7 days sampling after treatment respectively was selected to measure soluble sugar, soluble protein, proline, MDA, O₂^-, H₂O₂, stomatal density, photosynthetic pigments and chlorophyll fluorescence parameters. The results showed that, NO donor SNP could significantly increase the plant height of wheat-seedling after UV-B stress and soluble sugar, soluble protein and proline contents in leaves increased by 19.69%, 15.25% and 41.36%. At the same time, stomatal density of leaf positive surface increased by 10.34%. SNP could make photosynthetic pigments contents (chlorophyll and carotenoid) improve by 12.43% and 5.99%, and SNP could significantly raise their chlorophyll fluorescence parameter Fv/Fm and Fv/F0 by 0.5% and 5.16% compared with CK. Meanwhile, SNP could respectively decrease the level of MDA and H₂O₂ by 27.30% and 74.92% compared with CK and O₂^- producing rate significantly reduced by 24.47% . Related physiological indexes material content increase was needed to response to the wheat seedling damage under UV-B stress. While appropriate concentration of exogenous NO (0.1 mmol/L) could alleviate wheat seedling damage effect under UV-B stress. Consequently, exogenous NO could enhance wheat stress adaptive capacity under UV-B stress.

刘福霞帖海龙高丽丽韩榕. 外源NO对UV-B胁迫下小麦幼苗生长、活性氧组分和光合特性的影响[J]. 中国农学通报, 2013, 29(9): 43-50.

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