低温和短日照诱导白纹伊蚊滞育的代谢适应研究

doi:10.11853/j.issn.1003.8280.2024.02.003

摘要/Abstract

摘要： 目的检测白纹伊蚊滞育期间成蚊及卵内总糖、糖原、总胆固醇、甘油三酯、山梨醇、海藻糖指标含量的变化,为白纹伊蚊的滞育生理学研究提供理论基础。方法在实验室条件下,通过设置短日照和低温诱导白纹伊蚊滞育,取产卵后雌蚊及滞育维持期蚊卵,对总糖、糖原、海藻糖、甘油三酯、总胆固醇、山梨醇等生化物质进行提取,通过酶标仪检测其含量。运用GraphPad 8.0软件对实验数据进行非配对t检验统计分析。结果在滞育诱导条件下,白纹伊蚊成蚊总糖、糖原、海藻糖、山梨醇、甘油三酯、总胆固醇含量分别为(21.60±2.21)、(28.23±0.16)、(6.89±0.28)、(21.71±3.89)、(52.54±4.98) mg/g和(19.08±1.68) μmol/g,总糖、糖原、山梨醇较正常发育组高(均P<0.05)。蚊卵内总糖、糖原、海藻糖、总胆固醇生化指标含量分别为(103.94±8.06)、(8.61±1.54)、(1.35±0.07) mg/g和(13.58±0.28) μmol/g,均高于正常发育组(均P<0.01)。结论白纹伊蚊在滞育诱导条件下,卵内及成蚊体内生化指标发生不同程度的升高。总糖、糖原、海藻糖、总胆固醇等生化指标在蚊虫滞育生理中的作用在接下来的实验中可以进一步研究。

关键词: 滞育, 白纹伊蚊, 滞育卵, 代谢

Abstract: Objective To detect changes in the content of total sugar, glycogen, total cholesterol, triglyceride, sorbitol, and trehalose during diapause in the adults and eggs of Aedes albopictus, and to provide a theoretical basis for the study of diapause physiology of Ae. albopictus.Methods Under laboratory conditions, diapause was induced in Ae. albopictus by short sunshine duration and low temperature, and collected female mosquitoes having laid eggs and eggs in the maintenance phase of diapause to measure the content of biochemical substances (total sugar, glycogen, trehalose, triglyceride, total cholesterol, and sorbitol) using a microplate reader. The experimental data were analyzed by the unpaired t-test with the use of GraphPad 8.0 software.Results In diapausing Ae. albopictus adults, the levels of total sugar, glycogen, trehalose,sorbitol,triglyceride, andtotal cholesterol were (21.60±2.21), (28.23±0.16), (6.89±0.28), (21.71±3.89), (52.54±4.98) mg/g和(19.08±1.68) μmol/g, respectively, among which total sugar, glycogen, and sorbitol levels were higher than those of the normal development group (all P<0.05). The levels of total sugar, glycogen, trehalose, and total cholesterol in diapausing eggs were (103.94±8.06), (8.61±1.54), (1.35±0.07) mg/g, and (13.58±0.28) μmol/g, respectively, all higher than those in the normal development group (all P<0.01).ConclusionsAe. albopictus adults and eggs have varying increases in biochemical indices under diapause induction conditions, in which the role of total sugar, glycogen, trehalose, and total cholesterol in dispause physiology in mosquitoes deserve further attention.

Key words: Diapause, Aedes albopictus, Diapause egg, Metabolism

中图分类号:

R384.1
Q965

王淼, 简藓懿, 刘庆, 梁国锐, 谢静文, 王明, 姜玉庭, 赵彤言, 郭晓霞. 低温和短日照诱导白纹伊蚊滞育的代谢适应研究[J]. 中国媒介生物学及控制杂志, 2024, 35(2): 145-149.

WANG Miao, JIAN Xian-yi, LIU Qing, LIANG Guo-rui, XIE Jing-wen, WANG Ming, JIANG Yu-ting, ZHAO Tong-yan, GUO Xiao-xia. Metabolic adaptation associated with diapause in Aedes albopictus induced by low temperature and short sunshine duration[J]. Chinese Journal of Vector Biology and Control, 2024, 35(2): 145-149.

参考文献

[1] Paupy C,Delatte H,Bagny L,et al.Aedes albopictus,an arbovirus vector:From the darkness to the light[J].Microbes Infect,2009,11(14/15):1177-1185.DOI:10.1016/j.micinf.2009.05.005.
[2] Bonizzoni M,Gasperi G,Chen XG,et al.The invasive mosquito species Aedes albopictus:Current knowledge and future perspectives[J].Trends Parasitol,2013,29(9):460-468.DOI:10.1016/j.pt.2013.07.003.
[3] Denlinger DL,Armbruster PA.Mosquito diapause[J].Annu Rev Entomol,2014,59:73-93.DOI:10.1146/annurev-ento-011613-162023.
[4] Xia D.Aedes albopictus diapause and its photoperiodic regulation mechanism[D].Guangzhou:Southern Medical University,2022.DOI:10.27003/d.cnki.gojyu.2018.000148.(in Chinese) 夏丹.白纹伊蚊滞育及其光周期调控机制[D].广州:南方医科大学,2022.DOI:10.27003/d.cnki.gojyu.2018.000148.
[5] Chen ZZ,Zhao N,Yin XC,et al.Physiological and biochemical changes in naturally overwintering adults of Chrysoperla sinica(Neuroptera:Chrysopidae) during diapause termination under two different photoperiods[J].Acta Entomol Sin,2013,56(2):120-130.DOI:10.16380/j.kcxb.2013.02.002.(in Chinese) 陈珍珍,赵楠,印象初,等.中华通草蛉自然越冬成虫在两种光周期下滞育解除过程中的生理生化变化[J].昆虫学报,2013,56(2):120-130.DOI:10.16380/j.kcxb.2013.02.002.
[6] Huang NN,Dai P,Fu YG,et al.Measurement of physiological indices of cold tolerance in Bactrocera cucurbitae(Coquillett)[J].Chin J Appl Entomol,2015,52(3):694-701.DOI:10.7679/j.issn.2095-1353.2015.081.(in Chinese) 黄娜娜,代鹏,符悦冠,等.瓜实蝇耐低温相关生理指标的测定[J].应用昆虫学报,2015,52(3):694-701.DOI:10.7679/j.issn.2095-1353.2015.081.
[7] Su TY,Wang MY,Wang ZW,et al.Dynamic variations of body weight,water component,lipids,proteins,and carbohydrates of Cluex pipiens pallens(Diptera:Culicidae) during hibernation[J].Chin J Vector Biol Control,1992,3(6):337-341.(in Chinese) 苏天运,王梅英,王仲文,等.淡色库蚊越冬过程中体重、含水量及脂、蛋白质、碳水化合物含量的动态变化[J].中国媒介生物学及控制杂志,1992,3(6):337-341.
[8] Benoit JB,Denlinger DL.Suppression of water loss during adult diapause in the northern house mosquito,Culex pipiens[J].J Exp Biol,2007,210(Pt 2):217-226.DOI:10.1242/jeb.02630.
[9] Hahn DA,Denlinger DL.Energetics of insect diapause[J].Annu Rev Entomol,2011,56:103-121.DOI:10.1146/annurev-ento-112408-085436.
[10] Zhang BG,Zang AM,Wang LL,et al.Dynamic changes in trehalose and trehalase levels in overwintering adults of Culex pipiens pallens Coquillett (Diptera:Culicidae)[J].J Pathog Biol,2018,13(12):1335-1337,1343.DOI:10.13350/j.cjpb.181208.(in Chinese) 张本光,臧爱梅,王利磊,等.淡色库蚊越冬蚊体内海藻糖和海藻糖酶含量的变化[J].中国病原生物学杂志,2018,13(12):1335-1337,1343.DOI:10.13350/j.cjpb.181208.
[11] Hanson SM,Craig GBJr.Cold acclimation,diapause,and geographic origin affect cold hardiness in eggs of Aedes albopictus(Diptera:Culicidae)[J].J Med Entomol,1994,31(2):192-201.DOI:10.1093/jmedent/31.2.192.
[12] Urbanski JM,Benoit JB,Michaud MR,et al.The molecular physiology of increased egg desiccation resistance during diapause in the invasive mosquito,Aedes albopictus[J].Proc Biol Sci,2010,277(1694):2683-2692.DOI:10.1098/rspb.2010.0362.
[13] Reynolds JA,Poelchau MF,Rahman Z,et al.Transcript profiling reveals mechanisms for lipid conservation during diapause in the mosquito,Aedes albopictus[J].J Insect Physiol,2012,58(7):966-973.DOI:10.1016/j.jinsphys.2012.04.013.
[14] Yu CH,Lu D,Lin RH,et al.Trehalose:The blood sugar in insects[J].Chin Bull Entomol,2008,45(5):832-837.DOI:10.3969/j.issn.0452-8255.2008.05.035.(in Chinese) 于彩虹,卢丹,林荣华,等.海藻糖:昆虫的血糖[J].昆虫知识,2008,45(5):832-837.DOI:10.3969/j.issn.0452-8255.2008.05.035.
[15] Zhu F,Li H,Wang Y,et al.Change of trehalose glycogen and polyol contents of the diapausing larvae of Mylabris phalerata(Pallas) (Coleoptera:Meloidae) at different diapausing stages[J].Acta Entomol Sin,2008,51(1):9-13.DOI:10.16380/j.kcxb.2008.01.004.(in Chinese) 朱芬,李红,王永,等.大斑芫菁滞育幼虫在滞育不同阶段体内糖类和醇类含量的变化[J].昆虫学报,2008,51(1):9-13.DOI:10.16380/j.kcxb.2008.01.004.
[16] Guo TT,Ling F,Zhang SY,et al.The super-cooling ability and biochemical substances in diapausing and non-diapausing larvae of Grapholitha molesta(Busck) (Lepidoptera:Tortricidae)[J].Chin J Appl Entomol,2013,50(6):1514-1518.DOI:10.7679/j.issn.2095-1353.2013.208.(in Chinese) 郭婷婷,凌飞,张顺益,等.梨小食心虫滞育与非滞育幼虫过冷却能力与体内主要生化物质含量[J].应用昆虫学报,2013,50(6):1514-1518.DOI:10.7679/j.issn.2095-1353.2013.208.
[17] Hahn DA,Denlinger DL.Meeting the energetic demands of insect diapause:Nutrient storage and utilization[J].J Insect Physiol,2007,53(8):760-773.DOI:10.1016/j.jinsphys.2007.03.018.
[18] Zhang YJ.Studies on the cold hardiness of over wintering larva of Chilo supperssalis(Walker) and its mechanism[D].Wuhan:Huazhong Agricultural University,2008.DOI:10.7666/d.Y1198219.(in Chinese) 张拥军.二化螟越冬幼虫耐寒性及其机理研究[D].武汉:华中农业大学,2008.DOI:10.7666/d.Y1198219.
[19] Li YY,Zhang LS,Chen HY,et al.Temperature and photoperiodic response of diapause induction in Aphidius gifuensis Ashmead[J].Chin J Appl Entomol,2013,50(3):718-726.(in Chinese) 李玉艳,张礼生,陈红印,等.烟蚜茧蜂Aphidius gifuensis Ashmead滞育诱导的温光周期反应[J].应用昆虫学报,2013,50(3):718-726.
[20] Lyu B,Sun M,Zhai YF,et al.Changes of cold tolerance and biochemical substances in diapause and post-diapause Orius sauteri[J].Chin J Biol Control,2020,36(6):885-890.DOI:10.16409/j.cnki.2095-039x.2020.06.022.(in Chinese) 吕兵,孙猛,翟一凡,等.东亚小花蝽滞育前后耐寒能力及体内生化物质变化[J].中国生物防治学报,2020,36(6):885-890.DOI:10.16409/j.cnki.2095-039x.2020.06.022.
[21] Ren XY,Qi XY,An T,et al.Research on the accumulation,transformation and regulation of nutrients in diapause insects[J].Chin J Appl Entomol,2016,53(4):685-695.DOI:10.7679/j.issn.20951353.2016.086.(in Chinese) 任小云,齐晓阳,安涛,等.滞育昆虫营养物质的积累、转化与调控[J].应用昆虫学报,2016,53(4):685-695.DOI:10.7679/j.issn.20951353.2016.086.
[22] Zhang B,Peng Y,Zhao XJ,et al.Emergence of the overwintering generation of peach fruit moth (Carposina sasakii) depends on diapause and spring soil temperatures[J].J Insect Physiol,2016,86:32-39.DOI:10.1016/j.jinsphys.2015.12.007.
[23] Wu DY.Study on the physiology of diapause in silkworm,Bombyx mori:Studies of the effects of diapause hormone on lipid and carbohydrate metabolism in silkworm eggs and of long-term storage of diapause silkworm eggs[D].Chongqing:Southwest Agricultural University,2002.(in Chinese) 吴大洋.家蚕滞育的生理学研究:滞育激素对家蚕卵脂质、碳水化合物代谢的影响与滞育卵长期保存[D].重庆:西南农业大学,2002.
[24] Arrese EL,Soulages JL.Insect fat body:Energy,metabolism,and regulation[J].Annu Rev Entomol,2010,55:207-225.DOI:10.1146/annurev-ento-112408-085356.
[25] Wipking W,Viebahn M,Neumann D.Oxygen consumption,water,lipid and glycogen content of early and late diapause and non-diapause larvae of the burnet moth Zygaena trifolii[J].J Insect Physiol,1995,41(1):47-56.DOI:10.1016/0022-1910(94) 00079-V.