草地学报 ›› 2023, Vol. 31 ›› Issue (2): 358-364.DOI: 10.11733/j.issn.1007-0435.2023.02.007

• 研究论文 • 上一篇    

碱胁迫下内生真菌对宿主布顿大麦显微结构的影响

龙凤, 户萌菲, 陈胜, 刘小珍, 陈水红   

  1. 塔里木大学生命科学与技术学院/塔里木盆地生物资源保护利用兵团重点实验室, 新疆 阿拉尔 843300
  • 收稿日期:2022-09-26 修回日期:2022-10-21 发布日期:2023-02-28
  • 通讯作者: 陈水红,E-mail:cshdky@126.com
  • 作者简介:龙凤(1995-),女,重庆人,硕士研究生,主要从事禾草内生真菌抗逆研究,E-mail:761455426@qq.com
  • 基金资助:
    国家自然科学基金项目(32260356;31660689);塔里木大学校长基金(TDZKZD202201)资助

Effects of Endophytic Fungi on the Anatomical Structure of the host Hordeum bogdanii under Alkali Stress

LONG Feng, HU Meng-fei, CHEN Sheng, LIU Xiao-zhen, CHEN Shui-hong   

  1. 1. School of life science and technology, Tarim University/Key Laboratory of Tarim Basin biological resources protection and utilization corps, Alar, Xinjiang 843300, China
  • Received:2022-09-26 Revised:2022-10-21 Published:2023-02-28

摘要: 植物的根、茎、叶具有各自的生理功能和适应环境的形态结构。本研究以带有Epichloё内生真菌的布顿大麦(Hordeum bogdanii)(E+)和不带内生真菌的布顿大麦(E-)为试验材料,用50 mmol·L-1和100 mmol·L-1的混合碱(Na2CO3∶NaHCO3=1∶1)处理21 d后分别观察植物根、茎、叶显微结构的变化。结果表明:碱胁迫导致根的表皮出现皱缩和破裂,减小了根的维管束面积,增加了导管直径、表皮和内皮层厚度,并且E+的皱缩程度小于E-。碱处理使E+植株茎的气腔直径降低,表皮厚度升高。碱胁迫减小了叶的维管束面积和导管直径,增大了泡状细胞面积,且E+的维管束面积、导管直径和泡状细胞面积大于相同处理条件下的E-植株。内生真菌通过改变宿主植物根、茎和叶的显微结构,促进宿主植物在碱胁迫中加快对水分和无机盐的吸收、增加对细胞的保护和减少水分的散失以及提高植物的气体交换能力。

关键词: 碱胁迫, 内生真菌, 布顿大麦, 显微结构

Abstract: The roots,stems and leaves of plants have their own physiological functions and morphological structures that adapt to the environment. In this study,Hordeum bogdanii (E+) with Epichloё endophytic fungi and H. bogdanii (E-) without endophytic fungi were used as experimental materials. After 21 day of treatment with 50 mmol·L-1 and 100 mmol·L-1 mixed alkali (Na2CO3∶NaHCO3=1∶1),the anatomical changes of plant roots,stems and leaves were observed. The results showed that alkali stress led to root epidermis shrinkage and rupture,reduced the root vascular bundle area,increased the vessel diameter,epidermis,and endodermis thickness. And the degree of shrinkage of E+ plants was less than that of E-plants. Alkali treatment reduced the diameter of the air cavity of the E+ plant stem and increased the thickness of the epidermis. Alkali stress reduced the vascular bundle area and vessel diameter of leaves and increased the area of bulliform cells. And the vascular bundle area,vessel diameter and bulliform cell area of E+ were larger than those of E-plants under the same treatment conditions. By changing the microscopic structure of the roots,stems and leaves of the host plants,endophytic fungi promoted the absorption of water and inorganic salts,increased the protection of cells,reduced water loss and improved the gas exchange ability of the host plants under alkali stress.

Key words: Alkali stress, Endophytic fungi, Hordeum bogdanii, Microstructure

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