磷添加条件下摩西球囊霉与禾草内生真菌对多年生黑麦草生长的影响

doi:10.11686/cyxb2017071

摘要/Abstract

摘要： 本试验探究了不添加磷(P₀)和添加50 mg/kg(P₅₀)条件下,丛枝菌根(arbuscular mycorrhizae, AM)真菌摩西球囊霉(Glomus mosseae)和禾草内生真菌对多年生黑麦草生长、养分吸收和磷酸酶活性的影响。多年生黑麦草分别由含有禾草内生真菌(E⁺)和不含禾草内生真菌(E^-)种子建植获得。结果表明:1)AM真菌与禾草内生真菌可显著影响多年生黑麦草的生物量(P<0.05),二者共同作用(AME⁺)时生物量最低。P₀处理,以AM真菌E^-植株生物量最高。P₅₀条件下,对照(NME^-)最高;2)P添加平均提高AM真菌的侵染率11.40%,P₀和P₅₀条件下E⁺植株较E^-植株的菌根侵染率分别低18.65%和11.77%;3)AM真菌未显著影响P吸收(P<0.05)。禾草内生真菌增加了植株N含量,而AM真菌侵染降低了E⁺植株N含量;4)P添加显著提高了磷酸酶活性(P<0.05),不同处理以AME⁺的磷酸酶活性最高。两种共生微生物在不同P条件下,单独作用均有利于植物生长,共同作用时因对光合产物存在竞争,并未协同促进植物生长和养分吸收。

Abstract: This study aimed to determine the effects of arbuscular mycorrhizae fungi Glomus mosseae and grass endophytes on the growth, nutrient absorption and phosphatase activities of Lolium perenne without phosphorus addition (P₀) and with 50 mg/kg phosphorus addition (P₅₀). Seeds of perennial ryegrass infected with grass endophyte (E⁺) and without grass endophyte (E^-) were used to establish plants with and without the endophytes. The results showed that: 1) AM fungi and grass endophytes had significant effects on the host biomass (P<0.05); plants infected by both AM fungi and grass endophytes (AME⁺) had the lowest biomass. Plants infected by only Glomus mosseae had the highest biomass under P₀, while plants free of the two symbiosis fungi reached their highest biomass under P₅₀. 2) Phosphorus addition increased mycorrhizal colonization of perennial ryegrass by 11.40%, while infection with grass endophytes inhibited plant mycorrhizal colonization by 18.65% and 11.77% under P₀ and P₅₀ respectively. 3) G. mosseae had no significant effect on the absorption of P (P<0.05). Grass endophytes increased the content of total N, while AM colonization decreased the total N of plants with grass endophytes. 4) Phosphorus addition significantly increased phosphatase activities (P<0.05). Plants infected by AM fungi and grass endophytes had the highest phosphatase activities across the treatments. Each of the two symbiosis fungi improved plant growth when taken alone; however, they showed competition on photosynthate and did not enhance plant growth and nutrition absorption when applied together.

郭艳娥, 王晓瑜, 高萍, 段廷玉. 磷添加条件下摩西球囊霉与禾草内生真菌对多年生黑麦草生长的影响[J]. 草业学报, 2017, 26(12): 160-169.

GUO Yan-E, WANG Xiao-Yu, GAO Ping, DUAN Ting-Yu. Effects of Glomus mosseae and grass endophytes on the growth of Lolium perenne under phosphorus addition[J]. Acta Prataculturae Sinica, 2017, 26(12): 160-169.

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