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Development of Triglyceride Biosensor Based on a Platinum Nano Particle and Polypyrrole Nano Composite Electrode

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Authors: Chauhan, Ruchika; Nagar, Bhawana; Solanki, P. R.; Basu, T.

Source: Materials Focus, Volume 2, Number 4, August 2013, pp. 316-323(8)

Publisher: American Scientific Publishers

DOI: https://doi.org/10.1166/mat.2013.1096

The PPY/PtNP/PPY/ITO (PtNP/PPY/ITO) multilayered nanocomposite based films electrochemically deposited onto indium tin oxide (ITO) coated glass plate have been utilized for covalent immobilization of lipase (LIP), via N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) for triglyceride detection using amperometric technique. We have characterized PPY/ITO, PtNP/PPY/ITO electrodes, and LIP/PtNP/PPY/ITO multilayered bioelectrode using Fourier transform infrared (FTIR), scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. The electrochemical response of LIP/PtNP/PPY/ITO multilayered bioelectrode towards triglyceride, investigated using CV studies, exhibits linearity, detection limit and shelf life as 50–500 mg/dL, 25 mg/dL and 9 weeks, respectively. The value of the apparent Michaelis–Menten constant (K app m ) obtained as 18.54 mg/dL (0.89 mM) for LIP/PtNP/PPY/ITO multilayered bioelectrode indicates high affinity of lipase with tributyrin. Attempts have been made to detect triglyceride concentration in serum samples.

Keywords: BIOSENSOR; LIPASE; NANO COMPOSITE; PLATINUM NANO PARTICLES; POLYPYRROLE; TRIGLYCERIDE

Document Type: Research Article

Publication date: 01 August 2013

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  • Materials Focus is a multidisciplinary peer-reviewed journal with a very wide-ranging coverage, consolidates fundamental and applied research aspects in all areas of science and engineering of novel advanced materials including their synthesis, processing, spectroscopy, properties and device applications. The journal publishes full research papers, timely review articles, and short communications of important new scientific findings, encompassing all fundamental and applied research aspects of emerging advanced materials.
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