Persistent Identifier
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doi:10.11588/data/ADYUNN |
Publication Date
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2024-02-16 |
Title
| Triggered contraction of self-assembled micron-scale DNA nanotube rings [Research Data] |
Author
| Illig, Maja (Center for Molecular Biology of Heidelberg University (ZMBH), Im Neuenheimer Feld 329, 69120 Heidelberg, Germany, Max Planck Institute for Medical Research, Biophysical Engineering Group, Jahnstraße 29, 69120 Heidelberg, Germany) - ORCID: 0000-0002-3484-256X
Jahnke, Kevin (Max Planck Institute for Medical Research, Biophysical Engineering Group, Jahnstraße 29, 69120 Heidelberg, Germany, Harvard University, School of Engineering and Applied Sciences (SEAS), Cambridge, MA, USA)
Weise, Lukas P. (TU Dortmund University, Department of Physics, Otto-Hahn-Str. 4, 44221 Dortmund, Germany)
Scheffold, Marlene (Max Planck Institute for Medical Research, Biophysical Engineering Group, Jahnstraße 29, 69120 Heidelberg, Germany)
Mersdorf, Ulrike (Max Planck Institute for Medical Research, Biophysical Engineering Group, Jahnstraße 29, 69120 Heidelberg, Germany)
Drechsler, Hauke (B CUBE - Center for Molecular Bioengineering and Cluster of Excellence Physics of Life, Technische Universität Dresden, Tatzberg 41, 01307 Dresden, Germany, Tübingen University, Center for Plant Molecular Biology (ZMBP), Auf der Morgenstelle 32, 72076 Tübingen, Germany)
Zhang, Yixin (B CUBE - Center for Molecular Bioengineering and Cluster of Excellence Physics of Life, Technische Universität Dresden, Tatzberg 41, 01307 Dresden, Germany)
Diez, Stefan (B CUBE - Center for Molecular Bioengineering and Cluster of Excellence Physics of Life, Technische Universität Dresden, Tatzberg 41, 01307 Dresden, Germany, Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany)
Kierfeld, Jan (TU Dortmund University, Department of Physics, Otto-Hahn-Str. 4, 44221 Dortmund, Germany)
Göpfrich, Kerstin (Center for Molecular Biology of Heidelberg University (ZMBH), Im Neuenheimer Feld 329, 69120 Heidelberg, Germany, Max Planck Institute for Medical Research, Biophysical Engineering Group, Jahnstraße 29, 69120 Heidelberg, Germany) - ORCID: 0000-0003-2115-3551 |
Point of Contact
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Illig, Maja (Center for Molecular Biology of Heidelberg University (ZMBH), Im Neuenheimer Feld 329, 69120 Heidelberg, Germany; Max Planck Institute for Medical Research, Biophysical Engineering Group, Jahnstraße 29, 69120 Heidelberg, Germany) |
Description
| Contractile rings formed from cytoskeletal filaments mediate the division of cells. Ring formation is induced by specific crosslinkers, while contraction is typically associated with motor protein activity. Here, we engineer DNA nanotubes as mimics of cytoskeletal filaments and a synthetic crosslinker based on a peptide-functionalized starPEG construct. The crosslinker induces bundling of ten to hundred individual DNA nanotubes. Importantly, the DNA nanotube bundles curve into closed micron-scale rings in a one-pot self-assembly process yielding several thousand rings per microliter. Coarse-grained molecular dynamics simulations reproduce detailed architectural properties of the DNA rings as observed by electron microscopy. Furthermore, theory and simulations predict DNA ring contraction – without motor proteins – upon increasing attraction or decreasing bending rigidity of the DNA nanotubes, yielding mechanistic insights into the parameter space relevant for efficient nanotube sliding. We experimentally realize a variation of these parameters by addition of molecular crowders or temperature increase, respectively. In agreement between simulation and experiment, we obtain ring contraction to less than half of the initial ring diameter. DNA-based contractile rings could be a future element of an artificial division machinery in synthetic cells or of contractile muscle-like materials. (2023-12-06) |
Subject
| Chemistry; Medicine, Health and Life Sciences; Physics |
Keyword
| Bottom-up synthetic biology
DNA nanotubes
Contractile rings
Coarse-grained MD simulations |
Funding Information
| Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy via the Excellence Cluster 3D Matter Made to Order: EXC-2082/1 -- 390761711
Hector Fellow Academy
Carl Zeiss Foundation
Joachim Herz Foundation
Alexander von Humboldt Foundation
Max Planck Society |