In applying the glulam timber to the large-sized structures, the new types of connections have been developed. In presence, there are a few joint systems using steel plates and bolts. However, those systems are not always adequately satisfied with durability. Therefore, the new joint system by prestressing was developed. In Nagano prefecture, the timber-concrete composite bridge was provided as the standard design of timber bridges, and the joint system is by prestressing. In case of concrete girder, work of prestress decrease by elastic strain, creep, and etc. However, timber-concrete composite girder is not cleared numerically. In this study, we discussed the effective prestress on timber-concrete composite girder based on time-dependent of prestress checked in existing bridge.