We previously reported that low-dose irradiation activated antioxidant functions and decreased lipid peroxide levels in some organs of mouse. Acetaldehyde, which is a metabolic product of alcohol, is one substance that reacts with glutathione (GSH). Alcohol administration leads to GSH depletion in liver; suggesting direct conjugation of GSH with acetaldehyde and reactive intermediates of alcohol oxidation. In this study, we examined the inhibitory effect of low-dose X-inhalation on chronic alcohol-induced damage of mouse liver. C57BL/6J mice (female, about 8 weeks old) were irradiated by sham or 0.5 Gy of X-ray, and fed for 2 weeks on a Lieber DeCarile liquid diet, which contains of 5 % alcohol. We investigated the various biochemical parameters, such as the activities of transaminase and antioxidative enzymes in these hepatopathic mice. In result, the activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in serum were significantly increased by alcohol intake, suggesting that alcohol intake induced by hepatopathy. Histological examination also showed hepatopathy. We will more report the effects of low-dose X-irradiation on chronic alcohol-induced damage using the other biochemical parameters.